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Proteomic analysis reveals changes in the proteome of human THP-1 macrophages infected with Paracoccidioides brasiliensis

Paracoccidioides spp. is the etiologic agent of Paracoccidioidomycosis (PCM), a systemic disease with wide distribution in Latin America. Macrophages are very important cells during the response to infection by P. brasiliensis. In this study, we performed a proteomic analysis to evaluate the consequ...

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Autores principales: de Figueiredo, Ana Marina Barroso, Moraes, Dayane, Bailão, Alexandre Melo, Rocha, Olivia Basso, Silva, Lana Ohara Souza, Ribeiro-Dias, Fátima, Soares, Célia Maria de Almeida
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10693345/
https://www.ncbi.nlm.nih.gov/pubmed/38045758
http://dx.doi.org/10.3389/fcimb.2023.1275954
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author de Figueiredo, Ana Marina Barroso
Moraes, Dayane
Bailão, Alexandre Melo
Rocha, Olivia Basso
Silva, Lana Ohara Souza
Ribeiro-Dias, Fátima
Soares, Célia Maria de Almeida
author_facet de Figueiredo, Ana Marina Barroso
Moraes, Dayane
Bailão, Alexandre Melo
Rocha, Olivia Basso
Silva, Lana Ohara Souza
Ribeiro-Dias, Fátima
Soares, Célia Maria de Almeida
author_sort de Figueiredo, Ana Marina Barroso
collection PubMed
description Paracoccidioides spp. is the etiologic agent of Paracoccidioidomycosis (PCM), a systemic disease with wide distribution in Latin America. Macrophages are very important cells during the response to infection by P. brasiliensis. In this study, we performed a proteomic analysis to evaluate the consequences of P. brasiliensis yeast cells on the human THP-1 macrophage proteome. We have identified 443 and 2247 upregulated or downregulated proteins, respectively, in macrophages co-cultured with yeast cells of P. brasiliensis in comparison to control macrophages unexposed to the fungus. Proteomic analysis revealed that interaction with P. brasiliensis caused metabolic changes in macrophages that drastically affected energy production pathways. In addition, these macrophages presented regulated many factors related to epigenetic modifications and gene transcription as well as a decrease of many proteins associated to the immune system activity. This is the first human macrophage proteome derived from interactions with P. brasiliensis, which contributes to elucidating the changes that occur during the host response to this fungus. Furthermore, it highlights proteins that may be targets for the development of new therapeutic approaches to PCM.
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spelling pubmed-106933452023-12-03 Proteomic analysis reveals changes in the proteome of human THP-1 macrophages infected with Paracoccidioides brasiliensis de Figueiredo, Ana Marina Barroso Moraes, Dayane Bailão, Alexandre Melo Rocha, Olivia Basso Silva, Lana Ohara Souza Ribeiro-Dias, Fátima Soares, Célia Maria de Almeida Front Cell Infect Microbiol Cellular and Infection Microbiology Paracoccidioides spp. is the etiologic agent of Paracoccidioidomycosis (PCM), a systemic disease with wide distribution in Latin America. Macrophages are very important cells during the response to infection by P. brasiliensis. In this study, we performed a proteomic analysis to evaluate the consequences of P. brasiliensis yeast cells on the human THP-1 macrophage proteome. We have identified 443 and 2247 upregulated or downregulated proteins, respectively, in macrophages co-cultured with yeast cells of P. brasiliensis in comparison to control macrophages unexposed to the fungus. Proteomic analysis revealed that interaction with P. brasiliensis caused metabolic changes in macrophages that drastically affected energy production pathways. In addition, these macrophages presented regulated many factors related to epigenetic modifications and gene transcription as well as a decrease of many proteins associated to the immune system activity. This is the first human macrophage proteome derived from interactions with P. brasiliensis, which contributes to elucidating the changes that occur during the host response to this fungus. Furthermore, it highlights proteins that may be targets for the development of new therapeutic approaches to PCM. Frontiers Media S.A. 2023-11-16 /pmc/articles/PMC10693345/ /pubmed/38045758 http://dx.doi.org/10.3389/fcimb.2023.1275954 Text en Copyright © 2023 de Figueiredo, Moraes, Bailão, Rocha, Silva, Ribeiro-Dias and Soares https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Cellular and Infection Microbiology
de Figueiredo, Ana Marina Barroso
Moraes, Dayane
Bailão, Alexandre Melo
Rocha, Olivia Basso
Silva, Lana Ohara Souza
Ribeiro-Dias, Fátima
Soares, Célia Maria de Almeida
Proteomic analysis reveals changes in the proteome of human THP-1 macrophages infected with Paracoccidioides brasiliensis
title Proteomic analysis reveals changes in the proteome of human THP-1 macrophages infected with Paracoccidioides brasiliensis
title_full Proteomic analysis reveals changes in the proteome of human THP-1 macrophages infected with Paracoccidioides brasiliensis
title_fullStr Proteomic analysis reveals changes in the proteome of human THP-1 macrophages infected with Paracoccidioides brasiliensis
title_full_unstemmed Proteomic analysis reveals changes in the proteome of human THP-1 macrophages infected with Paracoccidioides brasiliensis
title_short Proteomic analysis reveals changes in the proteome of human THP-1 macrophages infected with Paracoccidioides brasiliensis
title_sort proteomic analysis reveals changes in the proteome of human thp-1 macrophages infected with paracoccidioides brasiliensis
topic Cellular and Infection Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10693345/
https://www.ncbi.nlm.nih.gov/pubmed/38045758
http://dx.doi.org/10.3389/fcimb.2023.1275954
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