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Short-term transplantation effect of a tissue-engineered meniscus constructed using drilled allogeneic acellular meniscus and BMSCs

During the construction of tissue-engineered meniscus, the low porosity of extracellular matrix restricts the flow of nutrient solution and the migration and proliferation of cells, thus affecting the tissue remodeling after transplantation. In this study, the canine allogeneic meniscus was drilled...

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Autores principales: Dai, Pengxiu, Zou, Tong, Zhao, Wen, Lv, Yangou, Gao, Dengke, Ruan, Chenmei, Zhang, Xia, Zhang, Xinke, Zhang, Yihua
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10693448/
https://www.ncbi.nlm.nih.gov/pubmed/38046571
http://dx.doi.org/10.3389/fvets.2023.1266018
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author Dai, Pengxiu
Zou, Tong
Zhao, Wen
Lv, Yangou
Gao, Dengke
Ruan, Chenmei
Zhang, Xia
Zhang, Xinke
Zhang, Yihua
author_facet Dai, Pengxiu
Zou, Tong
Zhao, Wen
Lv, Yangou
Gao, Dengke
Ruan, Chenmei
Zhang, Xia
Zhang, Xinke
Zhang, Yihua
author_sort Dai, Pengxiu
collection PubMed
description During the construction of tissue-engineered meniscus, the low porosity of extracellular matrix restricts the flow of nutrient solution and the migration and proliferation of cells, thus affecting the tissue remodeling after transplantation. In this study, the canine allogeneic meniscus was drilled first and then decellularized. The drilled tissue-engineered menisci (Drilled Allogeneic Acellular Meniscus + Bone Marrow Mesenchymal Stem Cells, BMSCs) were transplanted into the knee joints of model dogs. On the basis of ensuring the mechanical properties, the number of the porosity and the cells implanted in allogeneic acellular meniscus was significantly increased. The expression levels of glycosaminoglycans and type II collagen in the drilled tissue-engineered meniscus were also improved. It was determined that the animals in the experimental group recovered well-compared with those in the control group. The graft surface was covered with new cartilage, the retraction degree was small, and the tissue remodeling was good. The surface wear of the femoral condyle and tibial plateau cartilage was light. The results of this study showed that increasing the porosity of allogeneic meniscus by drilling could not only maintain the mechanical properties of the meniscus and increase the number of implanted cells but also promote cell proliferation and differentiation. After transplantation, the drilled tissue-engineered meniscus provided a good remodeling effect in vivo and played a positive role in repairing meniscal injury, protecting articular cartilage and restoring knee joint function.
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spelling pubmed-106934482023-12-03 Short-term transplantation effect of a tissue-engineered meniscus constructed using drilled allogeneic acellular meniscus and BMSCs Dai, Pengxiu Zou, Tong Zhao, Wen Lv, Yangou Gao, Dengke Ruan, Chenmei Zhang, Xia Zhang, Xinke Zhang, Yihua Front Vet Sci Veterinary Science During the construction of tissue-engineered meniscus, the low porosity of extracellular matrix restricts the flow of nutrient solution and the migration and proliferation of cells, thus affecting the tissue remodeling after transplantation. In this study, the canine allogeneic meniscus was drilled first and then decellularized. The drilled tissue-engineered menisci (Drilled Allogeneic Acellular Meniscus + Bone Marrow Mesenchymal Stem Cells, BMSCs) were transplanted into the knee joints of model dogs. On the basis of ensuring the mechanical properties, the number of the porosity and the cells implanted in allogeneic acellular meniscus was significantly increased. The expression levels of glycosaminoglycans and type II collagen in the drilled tissue-engineered meniscus were also improved. It was determined that the animals in the experimental group recovered well-compared with those in the control group. The graft surface was covered with new cartilage, the retraction degree was small, and the tissue remodeling was good. The surface wear of the femoral condyle and tibial plateau cartilage was light. The results of this study showed that increasing the porosity of allogeneic meniscus by drilling could not only maintain the mechanical properties of the meniscus and increase the number of implanted cells but also promote cell proliferation and differentiation. After transplantation, the drilled tissue-engineered meniscus provided a good remodeling effect in vivo and played a positive role in repairing meniscal injury, protecting articular cartilage and restoring knee joint function. Frontiers Media S.A. 2023-11-17 /pmc/articles/PMC10693448/ /pubmed/38046571 http://dx.doi.org/10.3389/fvets.2023.1266018 Text en Copyright © 2023 Dai, Zou, Zhao, Lv, Gao, Ruan, Zhang, Zhang and Zhang. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Veterinary Science
Dai, Pengxiu
Zou, Tong
Zhao, Wen
Lv, Yangou
Gao, Dengke
Ruan, Chenmei
Zhang, Xia
Zhang, Xinke
Zhang, Yihua
Short-term transplantation effect of a tissue-engineered meniscus constructed using drilled allogeneic acellular meniscus and BMSCs
title Short-term transplantation effect of a tissue-engineered meniscus constructed using drilled allogeneic acellular meniscus and BMSCs
title_full Short-term transplantation effect of a tissue-engineered meniscus constructed using drilled allogeneic acellular meniscus and BMSCs
title_fullStr Short-term transplantation effect of a tissue-engineered meniscus constructed using drilled allogeneic acellular meniscus and BMSCs
title_full_unstemmed Short-term transplantation effect of a tissue-engineered meniscus constructed using drilled allogeneic acellular meniscus and BMSCs
title_short Short-term transplantation effect of a tissue-engineered meniscus constructed using drilled allogeneic acellular meniscus and BMSCs
title_sort short-term transplantation effect of a tissue-engineered meniscus constructed using drilled allogeneic acellular meniscus and bmscs
topic Veterinary Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10693448/
https://www.ncbi.nlm.nih.gov/pubmed/38046571
http://dx.doi.org/10.3389/fvets.2023.1266018
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