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Tulathromycin metaphylaxis increases nasopharyngeal isolation of multidrug resistant Mannheimia haemolytica in stocker heifers

Bovine respiratory disease (BRD) is a leading cause of disease in feedlot and stocker calves with Mannheimia haemolytica (MH) as one of the most common etiologies. One of the most effective means of controlling BRD is through metaphylaxis, which involves administering antimicrobials to all animals a...

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Autores principales: Crosby, William B., Karisch, Brandi B., Hiott, Lari M., Pinnell, Lee J., Pittman, Alexandra, Frye, Jonathan G., Jackson, Charlene R., Loy, John Dustin, Epperson, William B., Blanton, John, Capik, Sarah F., Morley, Paul S., Woolums, Amelia R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10694364/
http://dx.doi.org/10.3389/fvets.2023.1256997
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author Crosby, William B.
Karisch, Brandi B.
Hiott, Lari M.
Pinnell, Lee J.
Pittman, Alexandra
Frye, Jonathan G.
Jackson, Charlene R.
Loy, John Dustin
Epperson, William B.
Blanton, John
Capik, Sarah F.
Morley, Paul S.
Woolums, Amelia R.
author_facet Crosby, William B.
Karisch, Brandi B.
Hiott, Lari M.
Pinnell, Lee J.
Pittman, Alexandra
Frye, Jonathan G.
Jackson, Charlene R.
Loy, John Dustin
Epperson, William B.
Blanton, John
Capik, Sarah F.
Morley, Paul S.
Woolums, Amelia R.
author_sort Crosby, William B.
collection PubMed
description Bovine respiratory disease (BRD) is a leading cause of disease in feedlot and stocker calves with Mannheimia haemolytica (MH) as one of the most common etiologies. One of the most effective means of controlling BRD is through metaphylaxis, which involves administering antimicrobials to all animals at high risk of developing BRD. However, increasing prevalence of multidrug resistant (MDR) MH may reduce efficacy of metaphylaxis due to decreased susceptibility to drugs used for metaphylaxis. Primarily, this study aimed to determine the effect of tulathromycin metaphylaxis and subsequent BRD treatment on antimicrobial resistance (AMR) in MH isolated from stocker calves. Secondary objectives included evaluating the effect of metaphylaxis and treatment for BRD on animal health and comparing the genetic relationship of MH isolated. Crossbred beef heifers (n = 331, mean weight = 232, SD = 17.8 kg) at high risk for BRD were randomly assigned to receive tulathromycin metaphylaxis (META, n = 167) or not (NO META, n = 164). Nasopharyngeal swabs were collected for MH isolation, antimicrobial susceptibility testing and whole genome sequencing at arrival and 3 (WK3) and 10 (WK10) weeks later. Mixed-effects logistic regression was used to identify risk factors for isolation of MH and MDR MH (resistant to ≥3 antimicrobial drug classes) at 3 and 10 weeks, BRD morbidity, and crude mortality. Animals in the META group had higher odds of isolation of MDR MH at 3 weeks [OR (95% CI) = 13.08 (5–30.9), p < 0.0001] and 10 weeks [OR (95% CI) = 5.92 (1.34–26.14), p = 0.019] after arrival. There was no difference in risk of isolation of any MH (resistant or susceptible) between META and NO META groups at all timepoints. Animals in the NO META group had 3 times higher odds of being treated for BRD [WK3: OR (95% CI) = 3.07 (1.70–5.52), p = 0.0002; WK10: OR (95% CI) = 2.76 (1.59–4.80), p = 0.0002]. Antimicrobial resistance genes found within isolates were associated with integrative conjugative element (ICE) genes. Tulathromycin metaphylaxis increased risk of isolation of MDR MH and in this population, the increase in MDR MH appeared to be associated with ICE containing antimicrobial resistance genes for multiple antimicrobial classes. This may have important implications for future efficacy of antimicrobials for control and treatment of BRD.
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spelling pubmed-106943642023-12-05 Tulathromycin metaphylaxis increases nasopharyngeal isolation of multidrug resistant Mannheimia haemolytica in stocker heifers Crosby, William B. Karisch, Brandi B. Hiott, Lari M. Pinnell, Lee J. Pittman, Alexandra Frye, Jonathan G. Jackson, Charlene R. Loy, John Dustin Epperson, William B. Blanton, John Capik, Sarah F. Morley, Paul S. Woolums, Amelia R. Front Vet Sci Veterinary Science Bovine respiratory disease (BRD) is a leading cause of disease in feedlot and stocker calves with Mannheimia haemolytica (MH) as one of the most common etiologies. One of the most effective means of controlling BRD is through metaphylaxis, which involves administering antimicrobials to all animals at high risk of developing BRD. However, increasing prevalence of multidrug resistant (MDR) MH may reduce efficacy of metaphylaxis due to decreased susceptibility to drugs used for metaphylaxis. Primarily, this study aimed to determine the effect of tulathromycin metaphylaxis and subsequent BRD treatment on antimicrobial resistance (AMR) in MH isolated from stocker calves. Secondary objectives included evaluating the effect of metaphylaxis and treatment for BRD on animal health and comparing the genetic relationship of MH isolated. Crossbred beef heifers (n = 331, mean weight = 232, SD = 17.8 kg) at high risk for BRD were randomly assigned to receive tulathromycin metaphylaxis (META, n = 167) or not (NO META, n = 164). Nasopharyngeal swabs were collected for MH isolation, antimicrobial susceptibility testing and whole genome sequencing at arrival and 3 (WK3) and 10 (WK10) weeks later. Mixed-effects logistic regression was used to identify risk factors for isolation of MH and MDR MH (resistant to ≥3 antimicrobial drug classes) at 3 and 10 weeks, BRD morbidity, and crude mortality. Animals in the META group had higher odds of isolation of MDR MH at 3 weeks [OR (95% CI) = 13.08 (5–30.9), p < 0.0001] and 10 weeks [OR (95% CI) = 5.92 (1.34–26.14), p = 0.019] after arrival. There was no difference in risk of isolation of any MH (resistant or susceptible) between META and NO META groups at all timepoints. Animals in the NO META group had 3 times higher odds of being treated for BRD [WK3: OR (95% CI) = 3.07 (1.70–5.52), p = 0.0002; WK10: OR (95% CI) = 2.76 (1.59–4.80), p = 0.0002]. Antimicrobial resistance genes found within isolates were associated with integrative conjugative element (ICE) genes. Tulathromycin metaphylaxis increased risk of isolation of MDR MH and in this population, the increase in MDR MH appeared to be associated with ICE containing antimicrobial resistance genes for multiple antimicrobial classes. This may have important implications for future efficacy of antimicrobials for control and treatment of BRD. Frontiers Media S.A. 2023-11-20 /pmc/articles/PMC10694364/ http://dx.doi.org/10.3389/fvets.2023.1256997 Text en Copyright © 2023 Crosby, Karisch, Hiott, Pinnell, Pittman, Frye, Jackson, Loy, Epperson, Blanton, Capik, Morley and Woolums. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Veterinary Science
Crosby, William B.
Karisch, Brandi B.
Hiott, Lari M.
Pinnell, Lee J.
Pittman, Alexandra
Frye, Jonathan G.
Jackson, Charlene R.
Loy, John Dustin
Epperson, William B.
Blanton, John
Capik, Sarah F.
Morley, Paul S.
Woolums, Amelia R.
Tulathromycin metaphylaxis increases nasopharyngeal isolation of multidrug resistant Mannheimia haemolytica in stocker heifers
title Tulathromycin metaphylaxis increases nasopharyngeal isolation of multidrug resistant Mannheimia haemolytica in stocker heifers
title_full Tulathromycin metaphylaxis increases nasopharyngeal isolation of multidrug resistant Mannheimia haemolytica in stocker heifers
title_fullStr Tulathromycin metaphylaxis increases nasopharyngeal isolation of multidrug resistant Mannheimia haemolytica in stocker heifers
title_full_unstemmed Tulathromycin metaphylaxis increases nasopharyngeal isolation of multidrug resistant Mannheimia haemolytica in stocker heifers
title_short Tulathromycin metaphylaxis increases nasopharyngeal isolation of multidrug resistant Mannheimia haemolytica in stocker heifers
title_sort tulathromycin metaphylaxis increases nasopharyngeal isolation of multidrug resistant mannheimia haemolytica in stocker heifers
topic Veterinary Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10694364/
http://dx.doi.org/10.3389/fvets.2023.1256997
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