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Dual stop codon suppression in mammalian cells with genomically integrated genetic code expansion machinery

Stop codon suppression using dedicated tRNA/aminoacyl-tRNA synthetase (aaRS) pairs allows for genetically encoded, site-specific incorporation of non-canonical amino acids (ncAAs) as chemical handles for protein labeling and modification. Here, we demonstrate that piggyBac-mediated genomic integrati...

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Detalles Bibliográficos
Autores principales:	Meineke, Birthe, Heimgärtner, Johannes, Caridha, Rozina, Block, Matthias F., Kimler, Kyle J., Pires, Maria F., Landreh, Michael, Elsässer, Simon J.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Elsevier 2023
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10694491/ https://www.ncbi.nlm.nih.gov/pubmed/37935196 http://dx.doi.org/10.1016/j.crmeth.2023.100626

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