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A versatile pumpless multi-channel fluidics system for maintenance and real-time functional assessment of tissue and cells

To address the needs of the life sciences community and the pharmaceutical industry in pre-clinical drug development to both maintain and continuously assess tissue metabolism and function with simple and rapid systems, we improved on the initial BaroFuse to develop it into a fully functional, pumpl...

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Autores principales: Kamat, Varun, Grumbine, Matthew K., Bao, Khang, Mokate, Kedar, Khalil, Gamal, Cook, Daniel, Clearwater, Brandon, Hirst, Richard, Harman, Jarrod, Boeck, Myriam, Fu, Zhongjie, Smith, Lois E.H., Goswami, Moloy, Wubben, Thomas J., Walker, Emily M., Zhu, Jie, Soleimanpour, Scott A., Scarlett, Jarrad M., Robbings, Brian M., Hass, Daniel, Hurley, James B., Sweet, Ian R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10694526/
https://www.ncbi.nlm.nih.gov/pubmed/37963464
http://dx.doi.org/10.1016/j.crmeth.2023.100642
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author Kamat, Varun
Grumbine, Matthew K.
Bao, Khang
Mokate, Kedar
Khalil, Gamal
Cook, Daniel
Clearwater, Brandon
Hirst, Richard
Harman, Jarrod
Boeck, Myriam
Fu, Zhongjie
Smith, Lois E.H.
Goswami, Moloy
Wubben, Thomas J.
Walker, Emily M.
Zhu, Jie
Soleimanpour, Scott A.
Scarlett, Jarrad M.
Robbings, Brian M.
Hass, Daniel
Hurley, James B.
Sweet, Ian R.
author_facet Kamat, Varun
Grumbine, Matthew K.
Bao, Khang
Mokate, Kedar
Khalil, Gamal
Cook, Daniel
Clearwater, Brandon
Hirst, Richard
Harman, Jarrod
Boeck, Myriam
Fu, Zhongjie
Smith, Lois E.H.
Goswami, Moloy
Wubben, Thomas J.
Walker, Emily M.
Zhu, Jie
Soleimanpour, Scott A.
Scarlett, Jarrad M.
Robbings, Brian M.
Hass, Daniel
Hurley, James B.
Sweet, Ian R.
author_sort Kamat, Varun
collection PubMed
description To address the needs of the life sciences community and the pharmaceutical industry in pre-clinical drug development to both maintain and continuously assess tissue metabolism and function with simple and rapid systems, we improved on the initial BaroFuse to develop it into a fully functional, pumpless, scalable multi-channel fluidics instrument that continuously measures changes in oxygen consumption and other endpoints in response to test compounds. We and several other laboratories assessed it with a wide range of tissue types including retina, pancreatic islets, liver, and hypothalamus with both aqueous and gaseous test compounds. The setup time was less than an hour for all collaborating groups, and there was close agreement between data obtained from the different laboratories. This easy-to-use system reliably generates real-time metabolic and functional data from tissue and cells in response to test compounds that will address a critical need in basic and applied research.
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spelling pubmed-106945262023-12-05 A versatile pumpless multi-channel fluidics system for maintenance and real-time functional assessment of tissue and cells Kamat, Varun Grumbine, Matthew K. Bao, Khang Mokate, Kedar Khalil, Gamal Cook, Daniel Clearwater, Brandon Hirst, Richard Harman, Jarrod Boeck, Myriam Fu, Zhongjie Smith, Lois E.H. Goswami, Moloy Wubben, Thomas J. Walker, Emily M. Zhu, Jie Soleimanpour, Scott A. Scarlett, Jarrad M. Robbings, Brian M. Hass, Daniel Hurley, James B. Sweet, Ian R. Cell Rep Methods Article To address the needs of the life sciences community and the pharmaceutical industry in pre-clinical drug development to both maintain and continuously assess tissue metabolism and function with simple and rapid systems, we improved on the initial BaroFuse to develop it into a fully functional, pumpless, scalable multi-channel fluidics instrument that continuously measures changes in oxygen consumption and other endpoints in response to test compounds. We and several other laboratories assessed it with a wide range of tissue types including retina, pancreatic islets, liver, and hypothalamus with both aqueous and gaseous test compounds. The setup time was less than an hour for all collaborating groups, and there was close agreement between data obtained from the different laboratories. This easy-to-use system reliably generates real-time metabolic and functional data from tissue and cells in response to test compounds that will address a critical need in basic and applied research. Elsevier 2023-11-13 /pmc/articles/PMC10694526/ /pubmed/37963464 http://dx.doi.org/10.1016/j.crmeth.2023.100642 Text en © 2023 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Article
Kamat, Varun
Grumbine, Matthew K.
Bao, Khang
Mokate, Kedar
Khalil, Gamal
Cook, Daniel
Clearwater, Brandon
Hirst, Richard
Harman, Jarrod
Boeck, Myriam
Fu, Zhongjie
Smith, Lois E.H.
Goswami, Moloy
Wubben, Thomas J.
Walker, Emily M.
Zhu, Jie
Soleimanpour, Scott A.
Scarlett, Jarrad M.
Robbings, Brian M.
Hass, Daniel
Hurley, James B.
Sweet, Ian R.
A versatile pumpless multi-channel fluidics system for maintenance and real-time functional assessment of tissue and cells
title A versatile pumpless multi-channel fluidics system for maintenance and real-time functional assessment of tissue and cells
title_full A versatile pumpless multi-channel fluidics system for maintenance and real-time functional assessment of tissue and cells
title_fullStr A versatile pumpless multi-channel fluidics system for maintenance and real-time functional assessment of tissue and cells
title_full_unstemmed A versatile pumpless multi-channel fluidics system for maintenance and real-time functional assessment of tissue and cells
title_short A versatile pumpless multi-channel fluidics system for maintenance and real-time functional assessment of tissue and cells
title_sort versatile pumpless multi-channel fluidics system for maintenance and real-time functional assessment of tissue and cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10694526/
https://www.ncbi.nlm.nih.gov/pubmed/37963464
http://dx.doi.org/10.1016/j.crmeth.2023.100642
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