Protocol for an in vitro assay to study HIV-1 Tat methylation

A critical virus-encoded regulator of HIV-1 transcription is the Tat protein, which is required to potently activate transcription. Tat is regulated by a wide variety of post-translational modifications. This protocol describes an in vitro assay to study Tat methylation. We describe steps for incorp...

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Detalles Bibliográficos
Autores principales: Boehm, Daniela, Kaehlcke, Katrin, Schnoelzer, Martina, Ott, Melanie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10694577/
https://www.ncbi.nlm.nih.gov/pubmed/37979180
http://dx.doi.org/10.1016/j.xpro.2023.102687

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