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Vibrio cyclitrophicus phage encoding gene transfer agent fragment, representing a novel viral family

Vibrio is a prevalent bacterial genus in aquatic environments and exhibits diverse metabolic capabilities, playing a vital role in marine biogeochemical cycles. This study isolated a novel virus infecting Vibrio cyclitrophicus, vB_VviC_ZQ26, from coastal waters near Qingdao, China. The vB_VviC_ZQ26...

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Autores principales: Xiong, Yao, Ma, Keran, Zou, Xiao, Liang, Yantao, Zheng, Kaiyang, Wang, Tiancong, Zhang, Hong, Dong, Yue, Wang, Ziyue, Liu, Yundan, Shao, Hongbing, McMinn, Andrew, Wang, Min
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10694778/
https://www.ncbi.nlm.nih.gov/pubmed/37972855
http://dx.doi.org/10.1016/j.virusres.2023.199270
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author Xiong, Yao
Ma, Keran
Zou, Xiao
Liang, Yantao
Zheng, Kaiyang
Wang, Tiancong
Zhang, Hong
Dong, Yue
Wang, Ziyue
Liu, Yundan
Shao, Hongbing
McMinn, Andrew
Wang, Min
author_facet Xiong, Yao
Ma, Keran
Zou, Xiao
Liang, Yantao
Zheng, Kaiyang
Wang, Tiancong
Zhang, Hong
Dong, Yue
Wang, Ziyue
Liu, Yundan
Shao, Hongbing
McMinn, Andrew
Wang, Min
author_sort Xiong, Yao
collection PubMed
description Vibrio is a prevalent bacterial genus in aquatic environments and exhibits diverse metabolic capabilities, playing a vital role in marine biogeochemical cycles. This study isolated a novel virus infecting Vibrio cyclitrophicus, vB_VviC_ZQ26, from coastal waters near Qingdao, China. The vB_VviC_ZQ26 comprises a linear double-stranded DNA genome with a length of 42,982 bp and a G + C content of 43.21 %, encoding 72 putative open reading frames (ORFs). Transmission electron microscope characterization indicates a siphoviral-morphology of vB_VviC_ZQ26. Nucleic-acids-wide analysis indicates a tetranucleotide frequency deviation for genomic segments encoding putative gene transfer agent protein (GTA) and coil-containing protein, implying divergent origins occurred in different parts of viral genomes. Phylogenetic and genome-content-based analysis suggest that vB_VviC_ZQ26 represents a novel vibriophage-specific family designated as Coheviridae. From the result of biogeographic analysis, Coheviridae is mainly colonized in the temperate and tropical epipelagic zones. This study describes a novel vibriophage infecting V. cyclitrophicus, shedding light on the evolutionary divergence of different parts of the viral genome and its ecological footprint in marine environments.
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spelling pubmed-106947782023-12-05 Vibrio cyclitrophicus phage encoding gene transfer agent fragment, representing a novel viral family Xiong, Yao Ma, Keran Zou, Xiao Liang, Yantao Zheng, Kaiyang Wang, Tiancong Zhang, Hong Dong, Yue Wang, Ziyue Liu, Yundan Shao, Hongbing McMinn, Andrew Wang, Min Virus Res Article Vibrio is a prevalent bacterial genus in aquatic environments and exhibits diverse metabolic capabilities, playing a vital role in marine biogeochemical cycles. This study isolated a novel virus infecting Vibrio cyclitrophicus, vB_VviC_ZQ26, from coastal waters near Qingdao, China. The vB_VviC_ZQ26 comprises a linear double-stranded DNA genome with a length of 42,982 bp and a G + C content of 43.21 %, encoding 72 putative open reading frames (ORFs). Transmission electron microscope characterization indicates a siphoviral-morphology of vB_VviC_ZQ26. Nucleic-acids-wide analysis indicates a tetranucleotide frequency deviation for genomic segments encoding putative gene transfer agent protein (GTA) and coil-containing protein, implying divergent origins occurred in different parts of viral genomes. Phylogenetic and genome-content-based analysis suggest that vB_VviC_ZQ26 represents a novel vibriophage-specific family designated as Coheviridae. From the result of biogeographic analysis, Coheviridae is mainly colonized in the temperate and tropical epipelagic zones. This study describes a novel vibriophage infecting V. cyclitrophicus, shedding light on the evolutionary divergence of different parts of the viral genome and its ecological footprint in marine environments. Elsevier 2023-11-18 /pmc/articles/PMC10694778/ /pubmed/37972855 http://dx.doi.org/10.1016/j.virusres.2023.199270 Text en © 2023 The Author(s) https://creativecommons.org/licenses/by-nc/4.0/This is an open access article under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).
spellingShingle Article
Xiong, Yao
Ma, Keran
Zou, Xiao
Liang, Yantao
Zheng, Kaiyang
Wang, Tiancong
Zhang, Hong
Dong, Yue
Wang, Ziyue
Liu, Yundan
Shao, Hongbing
McMinn, Andrew
Wang, Min
Vibrio cyclitrophicus phage encoding gene transfer agent fragment, representing a novel viral family
title Vibrio cyclitrophicus phage encoding gene transfer agent fragment, representing a novel viral family
title_full Vibrio cyclitrophicus phage encoding gene transfer agent fragment, representing a novel viral family
title_fullStr Vibrio cyclitrophicus phage encoding gene transfer agent fragment, representing a novel viral family
title_full_unstemmed Vibrio cyclitrophicus phage encoding gene transfer agent fragment, representing a novel viral family
title_short Vibrio cyclitrophicus phage encoding gene transfer agent fragment, representing a novel viral family
title_sort vibrio cyclitrophicus phage encoding gene transfer agent fragment, representing a novel viral family
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10694778/
https://www.ncbi.nlm.nih.gov/pubmed/37972855
http://dx.doi.org/10.1016/j.virusres.2023.199270
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