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Transcriptional insights of citrus defense response against Diaporthe citri

Citrus melanose, caused by Diaporthe citri, is one of the most important and widespread fungal diseases of citrus. Previous studies demonstrated that the citrus host was able to trigger the defense response to restrict the spread of D. citri. However, the molecular mechanism underlying this defense...

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Autores principales: Li, Pudong, Xiao, Xiaoe, Wang, Jingrui, Niu, Fan, Huang, Jiangnan, Xie, Bianyue, Ye, Lu, Zhang, Chaofan, Wang, Dengliang, Wu, Qun, Zheng, Xueliang, Gai, Yunpeng, Li, Hongye, Jiao, Chen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10694908/
https://www.ncbi.nlm.nih.gov/pubmed/38044435
http://dx.doi.org/10.1186/s12870-023-04624-x
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author Li, Pudong
Xiao, Xiaoe
Wang, Jingrui
Niu, Fan
Huang, Jiangnan
Xie, Bianyue
Ye, Lu
Zhang, Chaofan
Wang, Dengliang
Wu, Qun
Zheng, Xueliang
Gai, Yunpeng
Li, Hongye
Jiao, Chen
author_facet Li, Pudong
Xiao, Xiaoe
Wang, Jingrui
Niu, Fan
Huang, Jiangnan
Xie, Bianyue
Ye, Lu
Zhang, Chaofan
Wang, Dengliang
Wu, Qun
Zheng, Xueliang
Gai, Yunpeng
Li, Hongye
Jiao, Chen
author_sort Li, Pudong
collection PubMed
description Citrus melanose, caused by Diaporthe citri, is one of the most important and widespread fungal diseases of citrus. Previous studies demonstrated that the citrus host was able to trigger the defense response to restrict the spread of D. citri. However, the molecular mechanism underlying this defense response has yet to be elucidated. Here, we used RNA-Seq to explore the gene expression pattern at the early (3 days post infection, dpi) and late (14 dpi) infection stages of citrus leaves in response to D. citri infection, and outlined the differences in transcriptional regulation associated with defense responses. The functional enrichment analysis indicated that the plant cell wall biogenesis was significantly induced at the early infection stage, while the callose deposition response was more active at the late infection stage. CYP83B1 genes of the cytochrome P450 family were extensively induced in the callus deposition-mediated defense response. Remarkably, the gene encoding pectin methylesterase showed the highest upregulation and was only found to be differentially expressed at the late infection stage. Genes involved in the synthesis and regulation of phytoalexin coumarin were effectively activated. F6’H1 and S8H, encoding key enzymes in the biosynthesis of coumarins and their derivatives, were more strongly expressed at the late infection stage than at the early infection stage. Collectively, our study profiled the response pattern of citrus leaves against D. citri infection and provided the transcriptional evidence to support the defense mechanism. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12870-023-04624-x.
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spelling pubmed-106949082023-12-05 Transcriptional insights of citrus defense response against Diaporthe citri Li, Pudong Xiao, Xiaoe Wang, Jingrui Niu, Fan Huang, Jiangnan Xie, Bianyue Ye, Lu Zhang, Chaofan Wang, Dengliang Wu, Qun Zheng, Xueliang Gai, Yunpeng Li, Hongye Jiao, Chen BMC Plant Biol Research Citrus melanose, caused by Diaporthe citri, is one of the most important and widespread fungal diseases of citrus. Previous studies demonstrated that the citrus host was able to trigger the defense response to restrict the spread of D. citri. However, the molecular mechanism underlying this defense response has yet to be elucidated. Here, we used RNA-Seq to explore the gene expression pattern at the early (3 days post infection, dpi) and late (14 dpi) infection stages of citrus leaves in response to D. citri infection, and outlined the differences in transcriptional regulation associated with defense responses. The functional enrichment analysis indicated that the plant cell wall biogenesis was significantly induced at the early infection stage, while the callose deposition response was more active at the late infection stage. CYP83B1 genes of the cytochrome P450 family were extensively induced in the callus deposition-mediated defense response. Remarkably, the gene encoding pectin methylesterase showed the highest upregulation and was only found to be differentially expressed at the late infection stage. Genes involved in the synthesis and regulation of phytoalexin coumarin were effectively activated. F6’H1 and S8H, encoding key enzymes in the biosynthesis of coumarins and their derivatives, were more strongly expressed at the late infection stage than at the early infection stage. Collectively, our study profiled the response pattern of citrus leaves against D. citri infection and provided the transcriptional evidence to support the defense mechanism. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12870-023-04624-x. BioMed Central 2023-12-04 /pmc/articles/PMC10694908/ /pubmed/38044435 http://dx.doi.org/10.1186/s12870-023-04624-x Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Li, Pudong
Xiao, Xiaoe
Wang, Jingrui
Niu, Fan
Huang, Jiangnan
Xie, Bianyue
Ye, Lu
Zhang, Chaofan
Wang, Dengliang
Wu, Qun
Zheng, Xueliang
Gai, Yunpeng
Li, Hongye
Jiao, Chen
Transcriptional insights of citrus defense response against Diaporthe citri
title Transcriptional insights of citrus defense response against Diaporthe citri
title_full Transcriptional insights of citrus defense response against Diaporthe citri
title_fullStr Transcriptional insights of citrus defense response against Diaporthe citri
title_full_unstemmed Transcriptional insights of citrus defense response against Diaporthe citri
title_short Transcriptional insights of citrus defense response against Diaporthe citri
title_sort transcriptional insights of citrus defense response against diaporthe citri
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10694908/
https://www.ncbi.nlm.nih.gov/pubmed/38044435
http://dx.doi.org/10.1186/s12870-023-04624-x
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