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Litsea glaucescens Kuth possesses bactericidal activity against Listeria monocytogenes

BACKGROUND: Litsea glaucencens Kuth is an aromatic plant used for food seasoning food and in Mexican traditional medicine. Among, L. glaucencens leaves properties, it has proven antibacterial activity which can be used against opportunistic pathogens like Listeria monocytogenes, a foodborne bacteria...

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Autores principales: Gress-Antonio, Carlos David, Rivero-Perez, Nallely, Marquina-Bahena, Silvia, Alvarez, Laura, Zaragoza-Bastida, Adrian, Martínez-Juárez, Víctor Manuel, Sosa-Gutierrez, Carolina G., Ocampo-López, Juan, Zepeda-Bastida, Armando, Ojeda-Ramírez, Deyanira
Formato: Online Artículo Texto
Lenguaje:English
Publicado: PeerJ Inc. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10695109/
http://dx.doi.org/10.7717/peerj.16522
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author Gress-Antonio, Carlos David
Rivero-Perez, Nallely
Marquina-Bahena, Silvia
Alvarez, Laura
Zaragoza-Bastida, Adrian
Martínez-Juárez, Víctor Manuel
Sosa-Gutierrez, Carolina G.
Ocampo-López, Juan
Zepeda-Bastida, Armando
Ojeda-Ramírez, Deyanira
author_facet Gress-Antonio, Carlos David
Rivero-Perez, Nallely
Marquina-Bahena, Silvia
Alvarez, Laura
Zaragoza-Bastida, Adrian
Martínez-Juárez, Víctor Manuel
Sosa-Gutierrez, Carolina G.
Ocampo-López, Juan
Zepeda-Bastida, Armando
Ojeda-Ramírez, Deyanira
author_sort Gress-Antonio, Carlos David
collection PubMed
description BACKGROUND: Litsea glaucencens Kuth is an aromatic plant used for food seasoning food and in Mexican traditional medicine. Among, L. glaucencens leaves properties, it has proven antibacterial activity which can be used against opportunistic pathogens like Listeria monocytogenes, a foodborne bacteria that is the causal agent of listeriosis, a disease that can be fatal in susceptible individuals. The aim of this work was to investigate the antibacterial activity of L. glaucescens Kuth leaf extracts against L. monocytogenes and to identify its bioactive components. MATERIAL AND METHODS: L. glaucences leaves were macerated with four solvents of different polarity (n-hexane, dichloromethane, ethyl acetate, and methanol). To determine the capacity to inhibit bacterial proliferation in vitro, agar diffusion and microdilution methods were used. Next, we determined the minimal bactericidal concentration (MBC). Finally, we determined the ratio of MBC/MIC. Metabolites present in the active methanolic extract from L. glaucescens Kuth (LgMeOH) were purified by normal-phase open column chromatography. The structure of the antibacterial metabolite was determined using nuclear magnetic resonance ((1)H, (13)C, COSY, HSQC) and by comparison with known compounds. RESULTS: The LgMeOH extract was used to purify the compound responsible for the observed antimicrobial activity. This compound was identified as 5,7-dihydroxyflavanone (pinocembrin) by analysis of its spectroscopic data and comparison with those described. The MIC and MBC values obtained for pinocembrin were 0.68 mg/mL, and the ratio MBC/MIC for both LgMeOH and pinocembrin was one, which indicates bactericidal activity. CONCLUSION: L. glaucences Kuth leaves and its metabolite pinocembrin can be used to treat listeriosis due the bactericidal activity against L. monocytogenes.
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spelling pubmed-106951092023-12-05 Litsea glaucescens Kuth possesses bactericidal activity against Listeria monocytogenes Gress-Antonio, Carlos David Rivero-Perez, Nallely Marquina-Bahena, Silvia Alvarez, Laura Zaragoza-Bastida, Adrian Martínez-Juárez, Víctor Manuel Sosa-Gutierrez, Carolina G. Ocampo-López, Juan Zepeda-Bastida, Armando Ojeda-Ramírez, Deyanira PeerJ Biochemistry BACKGROUND: Litsea glaucencens Kuth is an aromatic plant used for food seasoning food and in Mexican traditional medicine. Among, L. glaucencens leaves properties, it has proven antibacterial activity which can be used against opportunistic pathogens like Listeria monocytogenes, a foodborne bacteria that is the causal agent of listeriosis, a disease that can be fatal in susceptible individuals. The aim of this work was to investigate the antibacterial activity of L. glaucescens Kuth leaf extracts against L. monocytogenes and to identify its bioactive components. MATERIAL AND METHODS: L. glaucences leaves were macerated with four solvents of different polarity (n-hexane, dichloromethane, ethyl acetate, and methanol). To determine the capacity to inhibit bacterial proliferation in vitro, agar diffusion and microdilution methods were used. Next, we determined the minimal bactericidal concentration (MBC). Finally, we determined the ratio of MBC/MIC. Metabolites present in the active methanolic extract from L. glaucescens Kuth (LgMeOH) were purified by normal-phase open column chromatography. The structure of the antibacterial metabolite was determined using nuclear magnetic resonance ((1)H, (13)C, COSY, HSQC) and by comparison with known compounds. RESULTS: The LgMeOH extract was used to purify the compound responsible for the observed antimicrobial activity. This compound was identified as 5,7-dihydroxyflavanone (pinocembrin) by analysis of its spectroscopic data and comparison with those described. The MIC and MBC values obtained for pinocembrin were 0.68 mg/mL, and the ratio MBC/MIC for both LgMeOH and pinocembrin was one, which indicates bactericidal activity. CONCLUSION: L. glaucences Kuth leaves and its metabolite pinocembrin can be used to treat listeriosis due the bactericidal activity against L. monocytogenes. PeerJ Inc. 2023-12-01 /pmc/articles/PMC10695109/ http://dx.doi.org/10.7717/peerj.16522 Text en ©2023 Gress-Antonio et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited.
spellingShingle Biochemistry
Gress-Antonio, Carlos David
Rivero-Perez, Nallely
Marquina-Bahena, Silvia
Alvarez, Laura
Zaragoza-Bastida, Adrian
Martínez-Juárez, Víctor Manuel
Sosa-Gutierrez, Carolina G.
Ocampo-López, Juan
Zepeda-Bastida, Armando
Ojeda-Ramírez, Deyanira
Litsea glaucescens Kuth possesses bactericidal activity against Listeria monocytogenes
title Litsea glaucescens Kuth possesses bactericidal activity against Listeria monocytogenes
title_full Litsea glaucescens Kuth possesses bactericidal activity against Listeria monocytogenes
title_fullStr Litsea glaucescens Kuth possesses bactericidal activity against Listeria monocytogenes
title_full_unstemmed Litsea glaucescens Kuth possesses bactericidal activity against Listeria monocytogenes
title_short Litsea glaucescens Kuth possesses bactericidal activity against Listeria monocytogenes
title_sort litsea glaucescens kuth possesses bactericidal activity against listeria monocytogenes
topic Biochemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10695109/
http://dx.doi.org/10.7717/peerj.16522
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