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Blazed oblique plane microscopy reveals scale-invariant inference of brain-wide population activity

Due to the size and opacity of vertebrate brains, it has until now been impossible to simultaneously record neuronal activity at cellular resolution across the entire adult brain. As a result, scientists are forced to choose between cellular-resolution microscopy over limited fields-of-view or whole...

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Autores principales: Hoffmann, Maximilian, Henninger, Jörg, Veith, Johannes, Richter, Lars, Judkewitz, Benjamin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10695970/
https://www.ncbi.nlm.nih.gov/pubmed/38049412
http://dx.doi.org/10.1038/s41467-023-43741-x
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author Hoffmann, Maximilian
Henninger, Jörg
Veith, Johannes
Richter, Lars
Judkewitz, Benjamin
author_facet Hoffmann, Maximilian
Henninger, Jörg
Veith, Johannes
Richter, Lars
Judkewitz, Benjamin
author_sort Hoffmann, Maximilian
collection PubMed
description Due to the size and opacity of vertebrate brains, it has until now been impossible to simultaneously record neuronal activity at cellular resolution across the entire adult brain. As a result, scientists are forced to choose between cellular-resolution microscopy over limited fields-of-view or whole-brain imaging at coarse-grained resolution. Bridging the gap between these spatial scales of understanding remains a major challenge in neuroscience. Here, we introduce blazed oblique plane microscopy to perform brain-wide recording of neuronal activity at cellular resolution in an adult vertebrate. Contrary to common belief, we find that inferences of neuronal population activity are near-independent of spatial scale: a set of randomly sampled neurons has a comparable predictive power as the same number of coarse-grained macrovoxels. Our work thus links cellular resolution with brain-wide scope, challenges the prevailing view that macroscale methods are generally inferior to microscale techniques and underscores the value of multiscale approaches to studying brain-wide activity.
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spelling pubmed-106959702023-12-06 Blazed oblique plane microscopy reveals scale-invariant inference of brain-wide population activity Hoffmann, Maximilian Henninger, Jörg Veith, Johannes Richter, Lars Judkewitz, Benjamin Nat Commun Article Due to the size and opacity of vertebrate brains, it has until now been impossible to simultaneously record neuronal activity at cellular resolution across the entire adult brain. As a result, scientists are forced to choose between cellular-resolution microscopy over limited fields-of-view or whole-brain imaging at coarse-grained resolution. Bridging the gap between these spatial scales of understanding remains a major challenge in neuroscience. Here, we introduce blazed oblique plane microscopy to perform brain-wide recording of neuronal activity at cellular resolution in an adult vertebrate. Contrary to common belief, we find that inferences of neuronal population activity are near-independent of spatial scale: a set of randomly sampled neurons has a comparable predictive power as the same number of coarse-grained macrovoxels. Our work thus links cellular resolution with brain-wide scope, challenges the prevailing view that macroscale methods are generally inferior to microscale techniques and underscores the value of multiscale approaches to studying brain-wide activity. Nature Publishing Group UK 2023-12-04 /pmc/articles/PMC10695970/ /pubmed/38049412 http://dx.doi.org/10.1038/s41467-023-43741-x Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Hoffmann, Maximilian
Henninger, Jörg
Veith, Johannes
Richter, Lars
Judkewitz, Benjamin
Blazed oblique plane microscopy reveals scale-invariant inference of brain-wide population activity
title Blazed oblique plane microscopy reveals scale-invariant inference of brain-wide population activity
title_full Blazed oblique plane microscopy reveals scale-invariant inference of brain-wide population activity
title_fullStr Blazed oblique plane microscopy reveals scale-invariant inference of brain-wide population activity
title_full_unstemmed Blazed oblique plane microscopy reveals scale-invariant inference of brain-wide population activity
title_short Blazed oblique plane microscopy reveals scale-invariant inference of brain-wide population activity
title_sort blazed oblique plane microscopy reveals scale-invariant inference of brain-wide population activity
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10695970/
https://www.ncbi.nlm.nih.gov/pubmed/38049412
http://dx.doi.org/10.1038/s41467-023-43741-x
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