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Blazed oblique plane microscopy reveals scale-invariant inference of brain-wide population activity
Due to the size and opacity of vertebrate brains, it has until now been impossible to simultaneously record neuronal activity at cellular resolution across the entire adult brain. As a result, scientists are forced to choose between cellular-resolution microscopy over limited fields-of-view or whole...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10695970/ https://www.ncbi.nlm.nih.gov/pubmed/38049412 http://dx.doi.org/10.1038/s41467-023-43741-x |
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author | Hoffmann, Maximilian Henninger, Jörg Veith, Johannes Richter, Lars Judkewitz, Benjamin |
author_facet | Hoffmann, Maximilian Henninger, Jörg Veith, Johannes Richter, Lars Judkewitz, Benjamin |
author_sort | Hoffmann, Maximilian |
collection | PubMed |
description | Due to the size and opacity of vertebrate brains, it has until now been impossible to simultaneously record neuronal activity at cellular resolution across the entire adult brain. As a result, scientists are forced to choose between cellular-resolution microscopy over limited fields-of-view or whole-brain imaging at coarse-grained resolution. Bridging the gap between these spatial scales of understanding remains a major challenge in neuroscience. Here, we introduce blazed oblique plane microscopy to perform brain-wide recording of neuronal activity at cellular resolution in an adult vertebrate. Contrary to common belief, we find that inferences of neuronal population activity are near-independent of spatial scale: a set of randomly sampled neurons has a comparable predictive power as the same number of coarse-grained macrovoxels. Our work thus links cellular resolution with brain-wide scope, challenges the prevailing view that macroscale methods are generally inferior to microscale techniques and underscores the value of multiscale approaches to studying brain-wide activity. |
format | Online Article Text |
id | pubmed-10695970 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-106959702023-12-06 Blazed oblique plane microscopy reveals scale-invariant inference of brain-wide population activity Hoffmann, Maximilian Henninger, Jörg Veith, Johannes Richter, Lars Judkewitz, Benjamin Nat Commun Article Due to the size and opacity of vertebrate brains, it has until now been impossible to simultaneously record neuronal activity at cellular resolution across the entire adult brain. As a result, scientists are forced to choose between cellular-resolution microscopy over limited fields-of-view or whole-brain imaging at coarse-grained resolution. Bridging the gap between these spatial scales of understanding remains a major challenge in neuroscience. Here, we introduce blazed oblique plane microscopy to perform brain-wide recording of neuronal activity at cellular resolution in an adult vertebrate. Contrary to common belief, we find that inferences of neuronal population activity are near-independent of spatial scale: a set of randomly sampled neurons has a comparable predictive power as the same number of coarse-grained macrovoxels. Our work thus links cellular resolution with brain-wide scope, challenges the prevailing view that macroscale methods are generally inferior to microscale techniques and underscores the value of multiscale approaches to studying brain-wide activity. Nature Publishing Group UK 2023-12-04 /pmc/articles/PMC10695970/ /pubmed/38049412 http://dx.doi.org/10.1038/s41467-023-43741-x Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Hoffmann, Maximilian Henninger, Jörg Veith, Johannes Richter, Lars Judkewitz, Benjamin Blazed oblique plane microscopy reveals scale-invariant inference of brain-wide population activity |
title | Blazed oblique plane microscopy reveals scale-invariant inference of brain-wide population activity |
title_full | Blazed oblique plane microscopy reveals scale-invariant inference of brain-wide population activity |
title_fullStr | Blazed oblique plane microscopy reveals scale-invariant inference of brain-wide population activity |
title_full_unstemmed | Blazed oblique plane microscopy reveals scale-invariant inference of brain-wide population activity |
title_short | Blazed oblique plane microscopy reveals scale-invariant inference of brain-wide population activity |
title_sort | blazed oblique plane microscopy reveals scale-invariant inference of brain-wide population activity |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10695970/ https://www.ncbi.nlm.nih.gov/pubmed/38049412 http://dx.doi.org/10.1038/s41467-023-43741-x |
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