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Protective effects of niacin following high fat rich diet: an in-vivo and in-silico study

Niacin had long been understood as an antioxidant. There were reports that high fat diet (HFD) may cause psychological and physical impairments. The present study was aimed to experience the effect of Niacin on % growth rate, cumulative food intake, motor activity and anxiety profile, redox status,...

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Autores principales: Samad, Noreen, Manzoor, Natasha, Batool, Ammara, Noor, Aqsa, Khaliq, Saima, Aurangzeb, Sana, Bhatti, Sheraz Ahmed, Imran, Imran
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10696033/
https://www.ncbi.nlm.nih.gov/pubmed/38049514
http://dx.doi.org/10.1038/s41598-023-48566-8
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author Samad, Noreen
Manzoor, Natasha
Batool, Ammara
Noor, Aqsa
Khaliq, Saima
Aurangzeb, Sana
Bhatti, Sheraz Ahmed
Imran, Imran
author_facet Samad, Noreen
Manzoor, Natasha
Batool, Ammara
Noor, Aqsa
Khaliq, Saima
Aurangzeb, Sana
Bhatti, Sheraz Ahmed
Imran, Imran
author_sort Samad, Noreen
collection PubMed
description Niacin had long been understood as an antioxidant. There were reports that high fat diet (HFD) may cause psychological and physical impairments. The present study was aimed to experience the effect of Niacin on % growth rate, cumulative food intake, motor activity and anxiety profile, redox status, 5-HT metabolism and brain histopathology in rats. Rats were administered with Niacin at a dose of 50 mg/ml/kg body weight for 4 weeks following normal diet (ND) and HFD. Behavioral tests were performed after 4 weeks. Animals were sacrificed to collect brain samples. Biochemical, neurochemical and histopathological studies were performed. HFD increased food intake and body weight. The exploratory activity was reduced and anxiety like behavior was observed in HFD treated animals. Activity of antioxidant enzymes was decreased while oxidative stress marker and serotonin metabolism in the brain of rat were increased in HFD treated animals than ND fed rats. Morphology of the brain was also altered by HFD administration. Conversely, Niacin treated animals decreased food intake and % growth rate, increased exploratory activity, produced anxiolytic effects, decreased oxidative stress and increased antioxidant enzyme and 5-HT levels following HFD. Morphology of brain is also normalized by the treatment of Niacin following HFD. In-silico studies showed that Niacin has a potential binding affinity with degradative enzyme of 5-HT i.e. monoamine oxidase (MAO) A and B with an energy of ~ − 4.5 and − 5.0 kcal/mol respectively. In conclusion, the present study showed that Niacin enhanced motor activity, produced anxiolytic effect, and reduced oxidative stress, appetite, growth rate, increased antioxidant enzymes and normalized serotonin system and brain morphology following HFD intake. In-silico studies suggested that increase 5-HT was associated with the binding of MAO with Niacin subsequentially an inhibition of the degradation of monoamine. It is suggested that Niacin has a great antioxidant potential and could be a good therapy for the treatment of HFD induced obesity.
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spelling pubmed-106960332023-12-06 Protective effects of niacin following high fat rich diet: an in-vivo and in-silico study Samad, Noreen Manzoor, Natasha Batool, Ammara Noor, Aqsa Khaliq, Saima Aurangzeb, Sana Bhatti, Sheraz Ahmed Imran, Imran Sci Rep Article Niacin had long been understood as an antioxidant. There were reports that high fat diet (HFD) may cause psychological and physical impairments. The present study was aimed to experience the effect of Niacin on % growth rate, cumulative food intake, motor activity and anxiety profile, redox status, 5-HT metabolism and brain histopathology in rats. Rats were administered with Niacin at a dose of 50 mg/ml/kg body weight for 4 weeks following normal diet (ND) and HFD. Behavioral tests were performed after 4 weeks. Animals were sacrificed to collect brain samples. Biochemical, neurochemical and histopathological studies were performed. HFD increased food intake and body weight. The exploratory activity was reduced and anxiety like behavior was observed in HFD treated animals. Activity of antioxidant enzymes was decreased while oxidative stress marker and serotonin metabolism in the brain of rat were increased in HFD treated animals than ND fed rats. Morphology of the brain was also altered by HFD administration. Conversely, Niacin treated animals decreased food intake and % growth rate, increased exploratory activity, produced anxiolytic effects, decreased oxidative stress and increased antioxidant enzyme and 5-HT levels following HFD. Morphology of brain is also normalized by the treatment of Niacin following HFD. In-silico studies showed that Niacin has a potential binding affinity with degradative enzyme of 5-HT i.e. monoamine oxidase (MAO) A and B with an energy of ~ − 4.5 and − 5.0 kcal/mol respectively. In conclusion, the present study showed that Niacin enhanced motor activity, produced anxiolytic effect, and reduced oxidative stress, appetite, growth rate, increased antioxidant enzymes and normalized serotonin system and brain morphology following HFD intake. In-silico studies suggested that increase 5-HT was associated with the binding of MAO with Niacin subsequentially an inhibition of the degradation of monoamine. It is suggested that Niacin has a great antioxidant potential and could be a good therapy for the treatment of HFD induced obesity. Nature Publishing Group UK 2023-12-01 /pmc/articles/PMC10696033/ /pubmed/38049514 http://dx.doi.org/10.1038/s41598-023-48566-8 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Samad, Noreen
Manzoor, Natasha
Batool, Ammara
Noor, Aqsa
Khaliq, Saima
Aurangzeb, Sana
Bhatti, Sheraz Ahmed
Imran, Imran
Protective effects of niacin following high fat rich diet: an in-vivo and in-silico study
title Protective effects of niacin following high fat rich diet: an in-vivo and in-silico study
title_full Protective effects of niacin following high fat rich diet: an in-vivo and in-silico study
title_fullStr Protective effects of niacin following high fat rich diet: an in-vivo and in-silico study
title_full_unstemmed Protective effects of niacin following high fat rich diet: an in-vivo and in-silico study
title_short Protective effects of niacin following high fat rich diet: an in-vivo and in-silico study
title_sort protective effects of niacin following high fat rich diet: an in-vivo and in-silico study
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10696033/
https://www.ncbi.nlm.nih.gov/pubmed/38049514
http://dx.doi.org/10.1038/s41598-023-48566-8
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