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Fluorescence lifetime Hong-Ou-Mandel sensing
Fluorescence Lifetime Imaging Microscopy in the time domain is typically performed by recording the arrival time of photons either by using electronic time tagging or a gated detector. As such the temporal resolution is limited by the performance of the electronics to 100’s of picoseconds. Here, we...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10696080/ https://www.ncbi.nlm.nih.gov/pubmed/38049423 http://dx.doi.org/10.1038/s41467-023-43868-x |
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author | Lyons, Ashley Zickus, Vytautas Álvarez-Mendoza, Raúl Triggiani, Danilo Tamma, Vincenzo Westerberg, Niclas Tassieri, Manlio Faccio, Daniele |
author_facet | Lyons, Ashley Zickus, Vytautas Álvarez-Mendoza, Raúl Triggiani, Danilo Tamma, Vincenzo Westerberg, Niclas Tassieri, Manlio Faccio, Daniele |
author_sort | Lyons, Ashley |
collection | PubMed |
description | Fluorescence Lifetime Imaging Microscopy in the time domain is typically performed by recording the arrival time of photons either by using electronic time tagging or a gated detector. As such the temporal resolution is limited by the performance of the electronics to 100’s of picoseconds. Here, we demonstrate a fluorescence lifetime measurement technique based on photon-bunching statistics with a resolution that is only dependent on the duration of the reference photon or laser pulse, which can readily reach the 1–0.1 picosecond timescale. A range of fluorescent dyes having lifetimes spanning from 1.6 to 7 picoseconds have been here measured with only ~1 s measurement duration. We corroborate the effectiveness of the technique by measuring the Newtonian viscosity of glycerol/water mixtures by means of a molecular rotor having over an order of magnitude variability in lifetime, thus introducing a new method for contact-free nanorheology. Accessing fluorescence lifetime information at such high temporal resolution opens a doorway for a wide range of fluorescent markers to be adopted for studying yet unexplored fast biological processes, as well as fundamental interactions such as lifetime shortening in resonant plasmonic devices. |
format | Online Article Text |
id | pubmed-10696080 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-106960802023-12-06 Fluorescence lifetime Hong-Ou-Mandel sensing Lyons, Ashley Zickus, Vytautas Álvarez-Mendoza, Raúl Triggiani, Danilo Tamma, Vincenzo Westerberg, Niclas Tassieri, Manlio Faccio, Daniele Nat Commun Article Fluorescence Lifetime Imaging Microscopy in the time domain is typically performed by recording the arrival time of photons either by using electronic time tagging or a gated detector. As such the temporal resolution is limited by the performance of the electronics to 100’s of picoseconds. Here, we demonstrate a fluorescence lifetime measurement technique based on photon-bunching statistics with a resolution that is only dependent on the duration of the reference photon or laser pulse, which can readily reach the 1–0.1 picosecond timescale. A range of fluorescent dyes having lifetimes spanning from 1.6 to 7 picoseconds have been here measured with only ~1 s measurement duration. We corroborate the effectiveness of the technique by measuring the Newtonian viscosity of glycerol/water mixtures by means of a molecular rotor having over an order of magnitude variability in lifetime, thus introducing a new method for contact-free nanorheology. Accessing fluorescence lifetime information at such high temporal resolution opens a doorway for a wide range of fluorescent markers to be adopted for studying yet unexplored fast biological processes, as well as fundamental interactions such as lifetime shortening in resonant plasmonic devices. Nature Publishing Group UK 2023-12-04 /pmc/articles/PMC10696080/ /pubmed/38049423 http://dx.doi.org/10.1038/s41467-023-43868-x Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Lyons, Ashley Zickus, Vytautas Álvarez-Mendoza, Raúl Triggiani, Danilo Tamma, Vincenzo Westerberg, Niclas Tassieri, Manlio Faccio, Daniele Fluorescence lifetime Hong-Ou-Mandel sensing |
title | Fluorescence lifetime Hong-Ou-Mandel sensing |
title_full | Fluorescence lifetime Hong-Ou-Mandel sensing |
title_fullStr | Fluorescence lifetime Hong-Ou-Mandel sensing |
title_full_unstemmed | Fluorescence lifetime Hong-Ou-Mandel sensing |
title_short | Fluorescence lifetime Hong-Ou-Mandel sensing |
title_sort | fluorescence lifetime hong-ou-mandel sensing |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10696080/ https://www.ncbi.nlm.nih.gov/pubmed/38049423 http://dx.doi.org/10.1038/s41467-023-43868-x |
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