Protocol for measuring erythrocyte glutathione reductase activity coefficient to assess riboflavin status

Riboflavin (vitamin B2) is a component of the co-enzyme flavin adenine dinucleotide (FAD). The activity coefficient of erythrocyte glutathione reductase (EGRAC), a FAD-dependent enzyme, is a biomarker of riboflavin status. Here, we describe a protocol for measuring unstimulated (basal) and FAD-stimu...

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Detalles Bibliográficos
Autores principales: Parkington, Damon A., Koulman, Albert, Jones, Kerry S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10696246/
http://dx.doi.org/10.1016/j.xpro.2023.102726
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author Parkington, Damon A.
Koulman, Albert
Jones, Kerry S.
author_facet Parkington, Damon A.
Koulman, Albert
Jones, Kerry S.
author_sort Parkington, Damon A.
collection PubMed
description Riboflavin (vitamin B2) is a component of the co-enzyme flavin adenine dinucleotide (FAD). The activity coefficient of erythrocyte glutathione reductase (EGRAC), a FAD-dependent enzyme, is a biomarker of riboflavin status. Here, we describe a protocol for measuring unstimulated (basal) and FAD-stimulated (activated) erythrocyte glutathione reductase activity to calculate EGRAC. We describe the steps for preparing washed red blood cells and hemolysates; preparing reagents; loading, incubating, and reading the 96-well plate; and calculating the results. For complete details on the use and execution of this protocol, please refer to Hess et al.(1)
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spelling pubmed-106962462023-12-06 Protocol for measuring erythrocyte glutathione reductase activity coefficient to assess riboflavin status Parkington, Damon A. Koulman, Albert Jones, Kerry S. STAR Protoc Protocol Riboflavin (vitamin B2) is a component of the co-enzyme flavin adenine dinucleotide (FAD). The activity coefficient of erythrocyte glutathione reductase (EGRAC), a FAD-dependent enzyme, is a biomarker of riboflavin status. Here, we describe a protocol for measuring unstimulated (basal) and FAD-stimulated (activated) erythrocyte glutathione reductase activity to calculate EGRAC. We describe the steps for preparing washed red blood cells and hemolysates; preparing reagents; loading, incubating, and reading the 96-well plate; and calculating the results. For complete details on the use and execution of this protocol, please refer to Hess et al.(1) Elsevier 2023-11-20 /pmc/articles/PMC10696246/ http://dx.doi.org/10.1016/j.xpro.2023.102726 Text en © 2023 The Authors https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Protocol
Parkington, Damon A.
Koulman, Albert
Jones, Kerry S.
Protocol for measuring erythrocyte glutathione reductase activity coefficient to assess riboflavin status
title Protocol for measuring erythrocyte glutathione reductase activity coefficient to assess riboflavin status
title_full Protocol for measuring erythrocyte glutathione reductase activity coefficient to assess riboflavin status
title_fullStr Protocol for measuring erythrocyte glutathione reductase activity coefficient to assess riboflavin status
title_full_unstemmed Protocol for measuring erythrocyte glutathione reductase activity coefficient to assess riboflavin status
title_short Protocol for measuring erythrocyte glutathione reductase activity coefficient to assess riboflavin status
title_sort protocol for measuring erythrocyte glutathione reductase activity coefficient to assess riboflavin status
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10696246/
http://dx.doi.org/10.1016/j.xpro.2023.102726
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