Protocol for establishing primary human lung organoid-derived air-liquid interface cultures from cryopreserved human lung tissue

Primary human lung organoid-derived air-liquid interface (ALI) cultures serve as a physiologically relevant model to study human airway epithelium in vitro. Here, we present a protocol for establishing these cultures from cryopreserved human lung tissue. We describe steps for lung tissue cryostorage...

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Detalles Bibliográficos
Autores principales: Castaneda, Diana Cadena, Jangra, Sonia, Yurieva, Marina, Martinek, Jan, Callender, Megan, Coxe, Matthew, Choi, Angela, García-Bernalt Diego, Juan, Wu, Te-Chia, Marches, Florentina, Chaussabel, Damien, García-Sastre, Adolfo, Schotsaert, Michael, Williams, Adam, Palucka, Karolina
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10696416/
http://dx.doi.org/10.1016/j.xpro.2023.102735

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