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Challenges and progress of neurodrug: bioactivities, production and delivery strategies of nerve growth factor protein

Nerve growth factor (NGF) is a vital cytokine that plays a crucial role in the development and regeneration of the nervous system. It has been extensively studied for its potential therapeutic applications in various neural diseases. However, as a protein drug, limited natural source seriously hinde...

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Autores principales: Zhou, Nan, Gu, TingWei, Xu, Yang, Liu, Yuda, Peng, LiHua
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10696794/
https://www.ncbi.nlm.nih.gov/pubmed/38049878
http://dx.doi.org/10.1186/s13036-023-00392-2
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author Zhou, Nan
Gu, TingWei
Xu, Yang
Liu, Yuda
Peng, LiHua
author_facet Zhou, Nan
Gu, TingWei
Xu, Yang
Liu, Yuda
Peng, LiHua
author_sort Zhou, Nan
collection PubMed
description Nerve growth factor (NGF) is a vital cytokine that plays a crucial role in the development and regeneration of the nervous system. It has been extensively studied for its potential therapeutic applications in various neural diseases. However, as a protein drug, limited natural source seriously hinders its translation and clinical applications. Conventional extraction of NGF from mouse submandibular glands has a very high cost and potentially induces immunogenicity; total synthesis and semi-synthesis methods are alternatives, but have difficulty in obtaining correct protein structure; gene engineering of plant cells is thought to be non-immunogenic, bioactive and economical. Meanwhile, large molecular weight, high polarity, and negative electrical charge make it difficult for NGF to cross the blood brain barrier to reach therapeutic targets. Current delivery strategies mainly depend on the adenovirus and cell biodelivery, but the safety and efficacy remain to be improved. New materials are widely investigated for the controllable, safe and precise delivery of NGF. This review illustrates physiological and therapeutic effects of NGF for various diseases. Moreover, new progress in production and delivery technologies for NGF are summarized. Bottlenecks encountered in the development of NGF as therapeutics are also discussed with the countermeasures proposed. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s13036-023-00392-2.
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spelling pubmed-106967942023-12-06 Challenges and progress of neurodrug: bioactivities, production and delivery strategies of nerve growth factor protein Zhou, Nan Gu, TingWei Xu, Yang Liu, Yuda Peng, LiHua J Biol Eng Review Nerve growth factor (NGF) is a vital cytokine that plays a crucial role in the development and regeneration of the nervous system. It has been extensively studied for its potential therapeutic applications in various neural diseases. However, as a protein drug, limited natural source seriously hinders its translation and clinical applications. Conventional extraction of NGF from mouse submandibular glands has a very high cost and potentially induces immunogenicity; total synthesis and semi-synthesis methods are alternatives, but have difficulty in obtaining correct protein structure; gene engineering of plant cells is thought to be non-immunogenic, bioactive and economical. Meanwhile, large molecular weight, high polarity, and negative electrical charge make it difficult for NGF to cross the blood brain barrier to reach therapeutic targets. Current delivery strategies mainly depend on the adenovirus and cell biodelivery, but the safety and efficacy remain to be improved. New materials are widely investigated for the controllable, safe and precise delivery of NGF. This review illustrates physiological and therapeutic effects of NGF for various diseases. Moreover, new progress in production and delivery technologies for NGF are summarized. Bottlenecks encountered in the development of NGF as therapeutics are also discussed with the countermeasures proposed. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s13036-023-00392-2. BioMed Central 2023-12-04 /pmc/articles/PMC10696794/ /pubmed/38049878 http://dx.doi.org/10.1186/s13036-023-00392-2 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Review
Zhou, Nan
Gu, TingWei
Xu, Yang
Liu, Yuda
Peng, LiHua
Challenges and progress of neurodrug: bioactivities, production and delivery strategies of nerve growth factor protein
title Challenges and progress of neurodrug: bioactivities, production and delivery strategies of nerve growth factor protein
title_full Challenges and progress of neurodrug: bioactivities, production and delivery strategies of nerve growth factor protein
title_fullStr Challenges and progress of neurodrug: bioactivities, production and delivery strategies of nerve growth factor protein
title_full_unstemmed Challenges and progress of neurodrug: bioactivities, production and delivery strategies of nerve growth factor protein
title_short Challenges and progress of neurodrug: bioactivities, production and delivery strategies of nerve growth factor protein
title_sort challenges and progress of neurodrug: bioactivities, production and delivery strategies of nerve growth factor protein
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10696794/
https://www.ncbi.nlm.nih.gov/pubmed/38049878
http://dx.doi.org/10.1186/s13036-023-00392-2
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