Cargando…

Polyethylene glycol precipitation is an efficient method to obtain extracellular vesicle-depleted fetal bovine serum

Mesenchymal stromal/stem cell derived-extracellular vesicles (MSC-EVs) have gained interest as drug delivery nanoparticles, having immunoregulatory and potentiating tissue repair property. To maintain growth of MSCs and obtain pure MSC-derived EVs, the culture media should contain fetal bovine serum...

Descripción completa

Detalles Bibliográficos
Autores principales:	Wang, Peng, Arntz, Onno J., Husch, Johanna F. A., der Kraan P. M., Van, van den Beucken, Jeroen J. J. P., van de Loo, Fons A. J.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Public Library of Science 2023
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10697576/ http://dx.doi.org/10.1371/journal.pone.0295076

Ejemplares similares

Bovine Milk‐Derived Extracellular Vesicles Inhibit Catabolic and Inflammatory Processes in Cartilage from Osteoarthritis Patients
por: Pieters, Bartijn C. H., et al.
Publicado: (2022)

Concentration of infectious SARS-CoV-2 by polyethylene glycol precipitation
por: Alexander, Marina R., et al.
Publicado: (2020)

The validation of macroprolactin analysis by polyethylene glycol precipitation using Fujirebio Lumipulse
por: Vermue, Febe C., et al.
Publicado: (2022)

Coculture with monocytes/macrophages modulates osteogenic differentiation of adipose‐derived mesenchymal stromal cells on poly(lactic‐co‐glycolic) acid/polycaprolactone scaffolds
por: Tang, Hongbo, et al.
Publicado: (2019)

Ultrafiltration – an alternative method to polyethylene glycol precipitation for macroprolactin detection
por: Beda-Maluga, Karolina, et al.
Publicado: (2015)

Polyethylene Glycol 3350 With Electrolytes Versus Polyethylene Glycol 4000 for Constipation: A Randomized, Controlled Trial
por: Bekkali, Noor L.H., et al.
Publicado: (2018)

Water on hydrophobic surfaces: mechanistic modeling of polyethylene glycol-induced protein precipitation
por: Großhans, Steffen, et al.
Publicado: (2018)

An optimized method for plasma extracellular vesicles isolation to exclude the copresence of biological drugs and plasma proteins which impairs their biological characterization
por: Arntz, Onno J., et al.
Publicado: (2020)

Various salts employed as precipitant in combination with polyethylene glycol in protein/detergent particle association
por: Odahara, Takayuki, et al.
Publicado: (2018)

Continuous capture of recombinant antibodies by ZnCl(2) precipitation without polyethylene glycol
por: Dutra, Gregory, et al.
Publicado: (2020)

Bactericidal activity of propylene glycol, glycerine, polyethylene glycol 400, and polyethylene glycol 1000 against selected microorganisms
por: Nalawade, Triveni Mohan, et al.
Publicado: (2015)

Reference ranges for the polyethylene glycol (PEG) precipitation activity (%PPA) of eight routine enzyme activities
por: Bürki, Carmen, et al.
Publicado: (2022)

Detection of macroenzymes: establishing upper reference limits for eight enzymes after polyethylene glycol precipitation
por: Dedeene, Lieselot, et al.
Publicado: (2022)

Synthesis of Stable Cholesteryl–Polyethylene Glycol–Peptide Conjugates with Non-Disperse Polyethylene Glycol Lengths
por: Cristóbal-Lecina, Edgar, et al.
Publicado: (2020)

Synthesis and Characterization of Polyethylene Glycol-Grafted Photoreactive Polyethylene Glycols for Antibiofouling Applications
por: Othman, Mahmoud H., et al.
Publicado: (2022)

A crucial role for tumor necrosis factor receptor 1 in synovial lining cells and the reticuloendothelial system in mediating experimental arthritis
por: Arntz, Onno J, et al.
Publicado: (2010)

IgY Technology: Extraction of Chicken Antibodies from Egg Yolk by Polyethylene Glycol (PEG) Precipitation
por: Pauly, Diana, et al.
Publicado: (2011)

Therapeutic Potential for Regulation of the Nuclear Factor Kappa-B Transcription Factor p65 to Prevent Cellular Senescence and Activation of Pro-Inflammatory in Mesenchymal Stem Cells
por: Mato-Basalo, Rocío, et al.
Publicado: (2021)

Polyethylene Glycol Camouflaged Earthworm Hemoglobin
por: Jani, Vivek P., et al.
Publicado: (2017)

Detection of Polyethylene Glycol Thyrotropin (TSH) Precipitable Percentage (Macro-TSH) in Patients with a History of Thyroid Cancer
por: Giusti, Massimo, et al.
Publicado: (2017)

Enhanced Detection of Low-Abundance Human Plasma Proteins by Integrating Polyethylene Glycol Fractionation and Immunoaffinity Depletion
por: Liu, Zhao, et al.
Publicado: (2016)

Altered biodistribution of an antibody--enzyme conjugate modified with polyethylene glycol.
por: Eno-Amooquaye, E. A., et al.
Publicado: (1996)

Pharmacokinetics, clearance, and biosafety of polyethylene glycol-coated hollow gold nanospheres
por: You, Jian, et al.
Publicado: (2014)

Polyethylene Glycol 20k. Does It Fluoresce?
por: Laatsch, Bethany F., et al.
Publicado: (2023)

Rheumatoid Arthritis Patients With Circulating Extracellular Vesicles Positive for IgM Rheumatoid Factor Have Higher Disease Activity
por: Arntz, Onno J., et al.
Publicado: (2018)

Diagnosis of insulin autoimmune syndrome using polyethylene glycol precipitation and gel filtration chromatography with ex vivo insulin exchange
por: Church, David, et al.
Publicado: (2016)

Polyethylene Glycol Precipitation to Avoid Misdiagnosis of Insulin Autoimmune Syndrome: A Case Report and Testing Pathway in Medical Laboratories
por: Zeng, Yuping, et al.
Publicado: (2022)

Extracellular Vesicles of Mesenchymal Stem Cells Are More Effectively Accessed through Polyethylene Glycol-Based Precipitation than by Ultracentrifugation
por: Jia, Lei, et al.
Publicado: (2022)

Innate Immunity and Sex: Distinct Inflammatory Profiles Associated with Murine Pain in Acute Synovitis
por: Valdrighi, Natália, et al.
Publicado: (2023)

Structural basis of polyethylene glycol recognition by antibody
por: Lee, Cheng-Chung, et al.
Publicado: (2020)

Polyethylene glycol as a cause of anaphylaxis
por: Wylon, Katharina, et al.
Publicado: (2016)

Novel Fluorinated Poly (Lactic-Co-Glycolic acid) (PLGA) and Polyethylene Glycol (PEG) Nanoparticles for Monitoring and Imaging in Osteoarthritis
por: Zerrillo, Luana, et al.
Publicado: (2021)

Polyethylene Glycol Fusion of Nerve Injuries: Review of the Technique and Clinical Applicability
por: Van Nest, Duncan S., et al.
Publicado: (2021)

Recurrent Rectal Prolapse Successfully Treated With Polyethylene Glycol
por: Lee, Regina J., et al.
Publicado: (2023)

Effects of polyethylene glycol content on the properties of a silk fibroin/nano-hydroxyapatite/polyethylene glycol electrospun scaffold
por: Qi, Qi, et al.
Publicado: (2019)

Intravenous Delivery of HIV-Based Lentiviral Vectors Preferentially Transduces F4/80+ and Ly-6C+ Cells in Spleen, Important Target Cells in Autoimmune Arthritis
por: van den Brand, Ben T., et al.
Publicado: (2013)

Influence of mesenchymal stem cell-derived extracellular vesicles in vitro and their role in ageing
por: Fafián-Labora, Juan, et al.
Publicado: (2020)

On the Behavior of the Ethylene Glycol Components of Polydisperse Polyethylene Glycol PEG200
por: Hoffmann, Markus M., et al.
Publicado: (2023)

Suppression of the inflammatory response by disease-inducible interleukin-10 gene therapy in a three-dimensional micromass model of the human synovial membrane
por: Broeren, Mathijs G. A., et al.
Publicado: (2016)

Commercial Cow Milk Contains Physically Stable Extracellular Vesicles Expressing Immunoregulatory TGF-β
por: Pieters, Bartijn C. H., et al.
Publicado: (2015)

Cannot write session to /tmp/vufind_sessions/sess_9shmedh8n0vd1amebo4m9td5t2