Correcting errors in synthetic DNA through consensus shuffling

Although efficient methods exist to assemble synthetic oligonucleotides into genes and genomes, these suffer from the presence of 1–3 random errors/kb of DNA. Here, we introduce a new method termed consensus shuffling and demonstrate its use to significantly reduce random errors in synthetic DNA. In...

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Detalles Bibliográficos
Autores principales: Binkowski, Brock F., Richmond, Kathryn E., Kaysen, James, Sussman, Michael R., Belshaw, Peter J.
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2005
Materias:
Methods Online
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1072806/
https://www.ncbi.nlm.nih.gov/pubmed/15800206
http://dx.doi.org/10.1093/nar/gni053

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1072806/
https://www.ncbi.nlm.nih.gov/pubmed/15800206
http://dx.doi.org/10.1093/nar/gni053

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