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Variation in the cortical area map of C57BL/6J and DBA/2J inbred mice predicts strain identity

BACKGROUND: Recent discoveries suggest that arealization of the mammalian cortical sheet develops in a manner consonant with principles established for embryonic patterning of the body. Signaling centers release morphogens that determine regional growth and tissue identity by regulating regional exp...

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Autores principales: Airey, David C, Robbins, Alicia I, Enzinger, Katherine M, Wu, Fangbai, Collins, Christine E
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2005
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1079866/
https://www.ncbi.nlm.nih.gov/pubmed/15774010
http://dx.doi.org/10.1186/1471-2202-6-18
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author Airey, David C
Robbins, Alicia I
Enzinger, Katherine M
Wu, Fangbai
Collins, Christine E
author_facet Airey, David C
Robbins, Alicia I
Enzinger, Katherine M
Wu, Fangbai
Collins, Christine E
author_sort Airey, David C
collection PubMed
description BACKGROUND: Recent discoveries suggest that arealization of the mammalian cortical sheet develops in a manner consonant with principles established for embryonic patterning of the body. Signaling centers release morphogens that determine regional growth and tissue identity by regulating regional expression of transcription factors. Research on mouse cortex has identified several candidate morphogens that affect anteroposterior or mediolateral cortical regionalization as well as mitogenesis. Inbred strains of laboratory mice can be exploited to study cortical area map formation if there are significant phenotypic differences with which to correlate gene polymorphism or expression data. Here we describe differences in the cortical area map of two commonly used inbred strains of laboratory mice, C57BL/6J and DBA/2J. Complete cortical hemispheres from adult mice were dissected and stained for the cytochrome oxidase enzyme in order to measure histochemically defined cortical areas. RESULTS: C57BL/6J has the larger neocortex, relatively larger primary visual cortex (V1), but relatively smaller posterior medial barrel subfield of the primary somatosensory cortex (PMBSF). The sample of C57BL/6J and DBA/2J mice can be discriminated with 90% accuracy on the basis of these three size dimensions. CONCLUSION: C57BL/6J and DBA/2J have markedly different cortical area maps, suggesting that inbred strains harbor enough phenotypic variation to encourage a forward genetic approach to understanding cortical development, complementing other approaches.
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spelling pubmed-10798662005-04-15 Variation in the cortical area map of C57BL/6J and DBA/2J inbred mice predicts strain identity Airey, David C Robbins, Alicia I Enzinger, Katherine M Wu, Fangbai Collins, Christine E BMC Neurosci Research Article BACKGROUND: Recent discoveries suggest that arealization of the mammalian cortical sheet develops in a manner consonant with principles established for embryonic patterning of the body. Signaling centers release morphogens that determine regional growth and tissue identity by regulating regional expression of transcription factors. Research on mouse cortex has identified several candidate morphogens that affect anteroposterior or mediolateral cortical regionalization as well as mitogenesis. Inbred strains of laboratory mice can be exploited to study cortical area map formation if there are significant phenotypic differences with which to correlate gene polymorphism or expression data. Here we describe differences in the cortical area map of two commonly used inbred strains of laboratory mice, C57BL/6J and DBA/2J. Complete cortical hemispheres from adult mice were dissected and stained for the cytochrome oxidase enzyme in order to measure histochemically defined cortical areas. RESULTS: C57BL/6J has the larger neocortex, relatively larger primary visual cortex (V1), but relatively smaller posterior medial barrel subfield of the primary somatosensory cortex (PMBSF). The sample of C57BL/6J and DBA/2J mice can be discriminated with 90% accuracy on the basis of these three size dimensions. CONCLUSION: C57BL/6J and DBA/2J have markedly different cortical area maps, suggesting that inbred strains harbor enough phenotypic variation to encourage a forward genetic approach to understanding cortical development, complementing other approaches. BioMed Central 2005-03-17 /pmc/articles/PMC1079866/ /pubmed/15774010 http://dx.doi.org/10.1186/1471-2202-6-18 Text en Copyright © 2005 Airey et al; licensee BioMed Central Ltd.
spellingShingle Research Article
Airey, David C
Robbins, Alicia I
Enzinger, Katherine M
Wu, Fangbai
Collins, Christine E
Variation in the cortical area map of C57BL/6J and DBA/2J inbred mice predicts strain identity
title Variation in the cortical area map of C57BL/6J and DBA/2J inbred mice predicts strain identity
title_full Variation in the cortical area map of C57BL/6J and DBA/2J inbred mice predicts strain identity
title_fullStr Variation in the cortical area map of C57BL/6J and DBA/2J inbred mice predicts strain identity
title_full_unstemmed Variation in the cortical area map of C57BL/6J and DBA/2J inbred mice predicts strain identity
title_short Variation in the cortical area map of C57BL/6J and DBA/2J inbred mice predicts strain identity
title_sort variation in the cortical area map of c57bl/6j and dba/2j inbred mice predicts strain identity
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1079866/
https://www.ncbi.nlm.nih.gov/pubmed/15774010
http://dx.doi.org/10.1186/1471-2202-6-18
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