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Transient increase of ATP as a response to temperature up-shift in Escherichia coli

BACKGROUND: Escherichia coli induces the heat shock response to a temperature up-shift which is connected to the synthesis of a characteristic set of proteins, including ATP dependent chaperones and proteases. Therefore the balance of the nucleotide pool is important for the adaptation and continuou...

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Autores principales: Soini, Jaakko, Falschlehner, Christina, Mayer, Christina, Böhm, Daniela, Weinel, Stefan, Panula, Johanna, Vasala, Antti, Neubauer, Peter
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2005
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1087501/
https://www.ncbi.nlm.nih.gov/pubmed/15804347
http://dx.doi.org/10.1186/1475-2859-4-9
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author Soini, Jaakko
Falschlehner, Christina
Mayer, Christina
Böhm, Daniela
Weinel, Stefan
Panula, Johanna
Vasala, Antti
Neubauer, Peter
author_facet Soini, Jaakko
Falschlehner, Christina
Mayer, Christina
Böhm, Daniela
Weinel, Stefan
Panula, Johanna
Vasala, Antti
Neubauer, Peter
author_sort Soini, Jaakko
collection PubMed
description BACKGROUND: Escherichia coli induces the heat shock response to a temperature up-shift which is connected to the synthesis of a characteristic set of proteins, including ATP dependent chaperones and proteases. Therefore the balance of the nucleotide pool is important for the adaptation and continuous function of the cell. Whereas it has been observed in eukaryotic cells, that the ATP level immediately decreased after the temperature shift, no data are available for E. coli about the adenosine nucleotide levels during the narrow time range of minutes after a temperature up-shift. RESULTS: The current study shows that a temperature up-shift is followed by a very fast significant transient increase of the cellular ATP concentration within the first minutes. This increase is connected to a longer lasting elevation of the cellular respiration and glucose uptake. Also the mRNA level of typical heat shock genes increases within only one minute after the heat-shock. CONCLUSION: The presented data prove the very fast response of E. coli to a heat-shock and that the initial response includes the increase of the ATP pool which is important to fulfil the need of the cell for new syntheses, as well as for the function of chaperones and proteases.
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spelling pubmed-10875012005-04-28 Transient increase of ATP as a response to temperature up-shift in Escherichia coli Soini, Jaakko Falschlehner, Christina Mayer, Christina Böhm, Daniela Weinel, Stefan Panula, Johanna Vasala, Antti Neubauer, Peter Microb Cell Fact Research BACKGROUND: Escherichia coli induces the heat shock response to a temperature up-shift which is connected to the synthesis of a characteristic set of proteins, including ATP dependent chaperones and proteases. Therefore the balance of the nucleotide pool is important for the adaptation and continuous function of the cell. Whereas it has been observed in eukaryotic cells, that the ATP level immediately decreased after the temperature shift, no data are available for E. coli about the adenosine nucleotide levels during the narrow time range of minutes after a temperature up-shift. RESULTS: The current study shows that a temperature up-shift is followed by a very fast significant transient increase of the cellular ATP concentration within the first minutes. This increase is connected to a longer lasting elevation of the cellular respiration and glucose uptake. Also the mRNA level of typical heat shock genes increases within only one minute after the heat-shock. CONCLUSION: The presented data prove the very fast response of E. coli to a heat-shock and that the initial response includes the increase of the ATP pool which is important to fulfil the need of the cell for new syntheses, as well as for the function of chaperones and proteases. BioMed Central 2005-04-01 /pmc/articles/PMC1087501/ /pubmed/15804347 http://dx.doi.org/10.1186/1475-2859-4-9 Text en Copyright © 2005 Soini et al; licensee BioMed Central Ltd.
spellingShingle Research
Soini, Jaakko
Falschlehner, Christina
Mayer, Christina
Böhm, Daniela
Weinel, Stefan
Panula, Johanna
Vasala, Antti
Neubauer, Peter
Transient increase of ATP as a response to temperature up-shift in Escherichia coli
title Transient increase of ATP as a response to temperature up-shift in Escherichia coli
title_full Transient increase of ATP as a response to temperature up-shift in Escherichia coli
title_fullStr Transient increase of ATP as a response to temperature up-shift in Escherichia coli
title_full_unstemmed Transient increase of ATP as a response to temperature up-shift in Escherichia coli
title_short Transient increase of ATP as a response to temperature up-shift in Escherichia coli
title_sort transient increase of atp as a response to temperature up-shift in escherichia coli
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1087501/
https://www.ncbi.nlm.nih.gov/pubmed/15804347
http://dx.doi.org/10.1186/1475-2859-4-9
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