CD34+ cells cultured in stem cell factor and interleukin-2 generate CD56+ cells with antiproliferative effects on tumor cell lines

In vitro stimulation of CD34+ cells with IL-2 induces NK cell differentiation. In order to define the stages of NK cell development, which influence their generation from CD34 cells, we cultured G-CSF mobilized peripheral blood CD34+ cells in the presence of stem cell factor and IL-2. After three we...

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Autores principales: Sconocchia, Giuseppe, Provenzano, Maurizio, Rezvani, Katayoun, Li, Jongming, Melenhorst, Jos, Hensel, Nancy, Barrett, A John
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2005
Materias:
Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1087889/
https://www.ncbi.nlm.nih.gov/pubmed/15831091
http://dx.doi.org/10.1186/1479-5876-3-15
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author Sconocchia, Giuseppe
Provenzano, Maurizio
Rezvani, Katayoun
Li, Jongming
Melenhorst, Jos
Hensel, Nancy
Barrett, A John
author_facet Sconocchia, Giuseppe
Provenzano, Maurizio
Rezvani, Katayoun
Li, Jongming
Melenhorst, Jos
Hensel, Nancy
Barrett, A John
author_sort Sconocchia, Giuseppe
collection PubMed
description In vitro stimulation of CD34+ cells with IL-2 induces NK cell differentiation. In order to define the stages of NK cell development, which influence their generation from CD34 cells, we cultured G-CSF mobilized peripheral blood CD34+ cells in the presence of stem cell factor and IL-2. After three weeks culture we found a diversity of CD56+ subsets which possessed granzyme A, but lacked the cytotoxic apparatus required for classical NK-like cytotoxicity. However, these CD56+ cells had the unusual property of inhibiting proliferation of K562 and P815 cell lines in a cell-contact dependent fashion.
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spelling pubmed-10878892005-04-30 CD34+ cells cultured in stem cell factor and interleukin-2 generate CD56+ cells with antiproliferative effects on tumor cell lines Sconocchia, Giuseppe Provenzano, Maurizio Rezvani, Katayoun Li, Jongming Melenhorst, Jos Hensel, Nancy Barrett, A John J Transl Med Research In vitro stimulation of CD34+ cells with IL-2 induces NK cell differentiation. In order to define the stages of NK cell development, which influence their generation from CD34 cells, we cultured G-CSF mobilized peripheral blood CD34+ cells in the presence of stem cell factor and IL-2. After three weeks culture we found a diversity of CD56+ subsets which possessed granzyme A, but lacked the cytotoxic apparatus required for classical NK-like cytotoxicity. However, these CD56+ cells had the unusual property of inhibiting proliferation of K562 and P815 cell lines in a cell-contact dependent fashion. BioMed Central 2005-04-14 /pmc/articles/PMC1087889/ /pubmed/15831091 http://dx.doi.org/10.1186/1479-5876-3-15 Text en Copyright © 2005 Sconocchia et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( (http://creativecommons.org/licenses/by/2.0) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research
Sconocchia, Giuseppe
Provenzano, Maurizio
Rezvani, Katayoun
Li, Jongming
Melenhorst, Jos
Hensel, Nancy
Barrett, A John
CD34+ cells cultured in stem cell factor and interleukin-2 generate CD56+ cells with antiproliferative effects on tumor cell lines
title CD34+ cells cultured in stem cell factor and interleukin-2 generate CD56+ cells with antiproliferative effects on tumor cell lines
title_full CD34+ cells cultured in stem cell factor and interleukin-2 generate CD56+ cells with antiproliferative effects on tumor cell lines
title_fullStr CD34+ cells cultured in stem cell factor and interleukin-2 generate CD56+ cells with antiproliferative effects on tumor cell lines
title_full_unstemmed CD34+ cells cultured in stem cell factor and interleukin-2 generate CD56+ cells with antiproliferative effects on tumor cell lines
title_short CD34+ cells cultured in stem cell factor and interleukin-2 generate CD56+ cells with antiproliferative effects on tumor cell lines
title_sort cd34+ cells cultured in stem cell factor and interleukin-2 generate cd56+ cells with antiproliferative effects on tumor cell lines
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1087889/
https://www.ncbi.nlm.nih.gov/pubmed/15831091
http://dx.doi.org/10.1186/1479-5876-3-15
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