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Morphological and growth altering effects of Cisplatin in C. albicans using fluorescence microscopy

Changes in morphology and growth curve of Candida albicans in response to treatment by Cisplatin has been studied using fluorescence staining with ethidium bromide. Treatment with Cisplatin was found to markedly inhibit hyphae and ovoid growth as revealed by ethidium bromide staining of drug treated...

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Autores principales: Kesavan, Chandrasekhar, Raghunathan, Malathi, Ganesan, Natarajan
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2005
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1090548/
https://www.ncbi.nlm.nih.gov/pubmed/15850483
http://dx.doi.org/10.1186/1476-0711-4-7
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author Kesavan, Chandrasekhar
Raghunathan, Malathi
Ganesan, Natarajan
author_facet Kesavan, Chandrasekhar
Raghunathan, Malathi
Ganesan, Natarajan
author_sort Kesavan, Chandrasekhar
collection PubMed
description Changes in morphology and growth curve of Candida albicans in response to treatment by Cisplatin has been studied using fluorescence staining with ethidium bromide. Treatment with Cisplatin was found to markedly inhibit hyphae and ovoid growth as revealed by ethidium bromide staining of drug treated cells. These changes were concomitant with inhibitory effects on the growth curve with respect to untreated cells Presence of Cisplatin not only caused suppression in the limiting values in the growth curve, but also caused a slight left shift in the EC50 values. Some of the ovoid cells undergoing poisoning with cisplatin were found to be unusually enlarged before undergoing their natural fate thus suggesting formation of similar cytotoxic end products with DNA.
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spelling pubmed-10905482005-05-07 Morphological and growth altering effects of Cisplatin in C. albicans using fluorescence microscopy Kesavan, Chandrasekhar Raghunathan, Malathi Ganesan, Natarajan Ann Clin Microbiol Antimicrob Research Changes in morphology and growth curve of Candida albicans in response to treatment by Cisplatin has been studied using fluorescence staining with ethidium bromide. Treatment with Cisplatin was found to markedly inhibit hyphae and ovoid growth as revealed by ethidium bromide staining of drug treated cells. These changes were concomitant with inhibitory effects on the growth curve with respect to untreated cells Presence of Cisplatin not only caused suppression in the limiting values in the growth curve, but also caused a slight left shift in the EC50 values. Some of the ovoid cells undergoing poisoning with cisplatin were found to be unusually enlarged before undergoing their natural fate thus suggesting formation of similar cytotoxic end products with DNA. BioMed Central 2005-04-25 /pmc/articles/PMC1090548/ /pubmed/15850483 http://dx.doi.org/10.1186/1476-0711-4-7 Text en Copyright © 2005 Kesavan et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( (http://creativecommons.org/licenses/by/2.0) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research
Kesavan, Chandrasekhar
Raghunathan, Malathi
Ganesan, Natarajan
Morphological and growth altering effects of Cisplatin in C. albicans using fluorescence microscopy
title Morphological and growth altering effects of Cisplatin in C. albicans using fluorescence microscopy
title_full Morphological and growth altering effects of Cisplatin in C. albicans using fluorescence microscopy
title_fullStr Morphological and growth altering effects of Cisplatin in C. albicans using fluorescence microscopy
title_full_unstemmed Morphological and growth altering effects of Cisplatin in C. albicans using fluorescence microscopy
title_short Morphological and growth altering effects of Cisplatin in C. albicans using fluorescence microscopy
title_sort morphological and growth altering effects of cisplatin in c. albicans using fluorescence microscopy
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1090548/
https://www.ncbi.nlm.nih.gov/pubmed/15850483
http://dx.doi.org/10.1186/1476-0711-4-7
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