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Expression of a novel carbonic anhydrase, CA XIII, in normal and neoplastic colorectal mucosa

BACKGROUND: Carbonic anhydrase (CA) isozymes may have an important role in cancer development. Some isozymes control pH homeostasis in tumors that appears to modulate the behaviour of cancer cells. CA XIII is the newest member of the CA gene family. It is a cytosolic isozyme which is expressed in a...

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Autores principales: Kummola, Laura, Hämäläinen, Jonna M, Kivelä, Jyrki, Kivelä, Antti J, Saarnio, Juha, Karttunen, Tuomo, Parkkila, Seppo
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2005
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1097719/
https://www.ncbi.nlm.nih.gov/pubmed/15836783
http://dx.doi.org/10.1186/1471-2407-5-41
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author Kummola, Laura
Hämäläinen, Jonna M
Kivelä, Jyrki
Kivelä, Antti J
Saarnio, Juha
Karttunen, Tuomo
Parkkila, Seppo
author_facet Kummola, Laura
Hämäläinen, Jonna M
Kivelä, Jyrki
Kivelä, Antti J
Saarnio, Juha
Karttunen, Tuomo
Parkkila, Seppo
author_sort Kummola, Laura
collection PubMed
description BACKGROUND: Carbonic anhydrase (CA) isozymes may have an important role in cancer development. Some isozymes control pH homeostasis in tumors that appears to modulate the behaviour of cancer cells. CA XIII is the newest member of the CA gene family. It is a cytosolic isozyme which is expressed in a number of normal tissues. The present study was designed to investigate CA XIII expression in prospectively collected colorectal tumor samples. METHODS: Both neoplastic and normal tissue specimens were obtained from the same patients. The analyses were performed using CA XIII-specific antibodies and an immunohistochemical staining method. For comparison, the tissue sections were immunostained for other cytosolic isozymes, CA I and II. RESULTS: The results indicated that the expression of CA XIII is down-regulated in tumor cells compared to the normal tissue. The lowest signal was detected in carcinoma samples. This pattern of expression was quite parallel for CA I and II. CONCLUSION: The down-regulation of cytosolic CA I, II and XIII in colorectal cancer may result from reduced levels of a common transcription factor or loss of closely linked CA1, CA2 and CA13 alleles on chromosome 8. Their possible role as tumor suppressors should be further evaluated.
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spelling pubmed-10977192005-05-12 Expression of a novel carbonic anhydrase, CA XIII, in normal and neoplastic colorectal mucosa Kummola, Laura Hämäläinen, Jonna M Kivelä, Jyrki Kivelä, Antti J Saarnio, Juha Karttunen, Tuomo Parkkila, Seppo BMC Cancer Research Article BACKGROUND: Carbonic anhydrase (CA) isozymes may have an important role in cancer development. Some isozymes control pH homeostasis in tumors that appears to modulate the behaviour of cancer cells. CA XIII is the newest member of the CA gene family. It is a cytosolic isozyme which is expressed in a number of normal tissues. The present study was designed to investigate CA XIII expression in prospectively collected colorectal tumor samples. METHODS: Both neoplastic and normal tissue specimens were obtained from the same patients. The analyses were performed using CA XIII-specific antibodies and an immunohistochemical staining method. For comparison, the tissue sections were immunostained for other cytosolic isozymes, CA I and II. RESULTS: The results indicated that the expression of CA XIII is down-regulated in tumor cells compared to the normal tissue. The lowest signal was detected in carcinoma samples. This pattern of expression was quite parallel for CA I and II. CONCLUSION: The down-regulation of cytosolic CA I, II and XIII in colorectal cancer may result from reduced levels of a common transcription factor or loss of closely linked CA1, CA2 and CA13 alleles on chromosome 8. Their possible role as tumor suppressors should be further evaluated. BioMed Central 2005-04-18 /pmc/articles/PMC1097719/ /pubmed/15836783 http://dx.doi.org/10.1186/1471-2407-5-41 Text en Copyright © 2005 Kummola et al; licensee BioMed Central Ltd.
spellingShingle Research Article
Kummola, Laura
Hämäläinen, Jonna M
Kivelä, Jyrki
Kivelä, Antti J
Saarnio, Juha
Karttunen, Tuomo
Parkkila, Seppo
Expression of a novel carbonic anhydrase, CA XIII, in normal and neoplastic colorectal mucosa
title Expression of a novel carbonic anhydrase, CA XIII, in normal and neoplastic colorectal mucosa
title_full Expression of a novel carbonic anhydrase, CA XIII, in normal and neoplastic colorectal mucosa
title_fullStr Expression of a novel carbonic anhydrase, CA XIII, in normal and neoplastic colorectal mucosa
title_full_unstemmed Expression of a novel carbonic anhydrase, CA XIII, in normal and neoplastic colorectal mucosa
title_short Expression of a novel carbonic anhydrase, CA XIII, in normal and neoplastic colorectal mucosa
title_sort expression of a novel carbonic anhydrase, ca xiii, in normal and neoplastic colorectal mucosa
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1097719/
https://www.ncbi.nlm.nih.gov/pubmed/15836783
http://dx.doi.org/10.1186/1471-2407-5-41
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