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Expression of a novel carbonic anhydrase, CA XIII, in normal and neoplastic colorectal mucosa
BACKGROUND: Carbonic anhydrase (CA) isozymes may have an important role in cancer development. Some isozymes control pH homeostasis in tumors that appears to modulate the behaviour of cancer cells. CA XIII is the newest member of the CA gene family. It is a cytosolic isozyme which is expressed in a...
Autores principales: | , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
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BioMed Central
2005
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1097719/ https://www.ncbi.nlm.nih.gov/pubmed/15836783 http://dx.doi.org/10.1186/1471-2407-5-41 |
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author | Kummola, Laura Hämäläinen, Jonna M Kivelä, Jyrki Kivelä, Antti J Saarnio, Juha Karttunen, Tuomo Parkkila, Seppo |
author_facet | Kummola, Laura Hämäläinen, Jonna M Kivelä, Jyrki Kivelä, Antti J Saarnio, Juha Karttunen, Tuomo Parkkila, Seppo |
author_sort | Kummola, Laura |
collection | PubMed |
description | BACKGROUND: Carbonic anhydrase (CA) isozymes may have an important role in cancer development. Some isozymes control pH homeostasis in tumors that appears to modulate the behaviour of cancer cells. CA XIII is the newest member of the CA gene family. It is a cytosolic isozyme which is expressed in a number of normal tissues. The present study was designed to investigate CA XIII expression in prospectively collected colorectal tumor samples. METHODS: Both neoplastic and normal tissue specimens were obtained from the same patients. The analyses were performed using CA XIII-specific antibodies and an immunohistochemical staining method. For comparison, the tissue sections were immunostained for other cytosolic isozymes, CA I and II. RESULTS: The results indicated that the expression of CA XIII is down-regulated in tumor cells compared to the normal tissue. The lowest signal was detected in carcinoma samples. This pattern of expression was quite parallel for CA I and II. CONCLUSION: The down-regulation of cytosolic CA I, II and XIII in colorectal cancer may result from reduced levels of a common transcription factor or loss of closely linked CA1, CA2 and CA13 alleles on chromosome 8. Their possible role as tumor suppressors should be further evaluated. |
format | Text |
id | pubmed-1097719 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2005 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-10977192005-05-12 Expression of a novel carbonic anhydrase, CA XIII, in normal and neoplastic colorectal mucosa Kummola, Laura Hämäläinen, Jonna M Kivelä, Jyrki Kivelä, Antti J Saarnio, Juha Karttunen, Tuomo Parkkila, Seppo BMC Cancer Research Article BACKGROUND: Carbonic anhydrase (CA) isozymes may have an important role in cancer development. Some isozymes control pH homeostasis in tumors that appears to modulate the behaviour of cancer cells. CA XIII is the newest member of the CA gene family. It is a cytosolic isozyme which is expressed in a number of normal tissues. The present study was designed to investigate CA XIII expression in prospectively collected colorectal tumor samples. METHODS: Both neoplastic and normal tissue specimens were obtained from the same patients. The analyses were performed using CA XIII-specific antibodies and an immunohistochemical staining method. For comparison, the tissue sections were immunostained for other cytosolic isozymes, CA I and II. RESULTS: The results indicated that the expression of CA XIII is down-regulated in tumor cells compared to the normal tissue. The lowest signal was detected in carcinoma samples. This pattern of expression was quite parallel for CA I and II. CONCLUSION: The down-regulation of cytosolic CA I, II and XIII in colorectal cancer may result from reduced levels of a common transcription factor or loss of closely linked CA1, CA2 and CA13 alleles on chromosome 8. Their possible role as tumor suppressors should be further evaluated. BioMed Central 2005-04-18 /pmc/articles/PMC1097719/ /pubmed/15836783 http://dx.doi.org/10.1186/1471-2407-5-41 Text en Copyright © 2005 Kummola et al; licensee BioMed Central Ltd. |
spellingShingle | Research Article Kummola, Laura Hämäläinen, Jonna M Kivelä, Jyrki Kivelä, Antti J Saarnio, Juha Karttunen, Tuomo Parkkila, Seppo Expression of a novel carbonic anhydrase, CA XIII, in normal and neoplastic colorectal mucosa |
title | Expression of a novel carbonic anhydrase, CA XIII, in normal and neoplastic colorectal mucosa |
title_full | Expression of a novel carbonic anhydrase, CA XIII, in normal and neoplastic colorectal mucosa |
title_fullStr | Expression of a novel carbonic anhydrase, CA XIII, in normal and neoplastic colorectal mucosa |
title_full_unstemmed | Expression of a novel carbonic anhydrase, CA XIII, in normal and neoplastic colorectal mucosa |
title_short | Expression of a novel carbonic anhydrase, CA XIII, in normal and neoplastic colorectal mucosa |
title_sort | expression of a novel carbonic anhydrase, ca xiii, in normal and neoplastic colorectal mucosa |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1097719/ https://www.ncbi.nlm.nih.gov/pubmed/15836783 http://dx.doi.org/10.1186/1471-2407-5-41 |
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