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Comparing protocols for preparation of DNA-free total yeast RNA suitable for RT-PCR

BACKGROUND: Preparation of RNA free from DNA is a critical step before performing RT-PCR assay. Total RNA isolated from several sources, including those obtained from Saccharomyces cerevisiae, using routine methodologies are frequently contaminated with DNA, which can give rise to amplification prod...

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Detalles Bibliográficos
Autores principales:	Del Aguila, Eduardo M, Dutra, Marcio B, Silva, Joab T, Paschoalin, Vânia MF
Formato:	Texto
Lenguaje:	English
Publicado:	BioMed Central 2005
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1097730/ https://www.ncbi.nlm.nih.gov/pubmed/15833107 http://dx.doi.org/10.1186/1471-2199-6-9

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