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Offset recombinant PCR: a simple but effective method for shuffling compact heterologous domains
DNA shuffling and other in vitro recombination strategies have proven highly effective at generating complex libraries for mutagenesis studies. While most recombination techniques employ DNA polymerases in part of a multi-step process, few seek to exploit the natural recombinogenic tendencies and ex...
| Autores principales: | , |
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| Formato: | Texto |
| Lenguaje: | English |
| Publicado: |
Oxford University Press
2005
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1130489/ https://www.ncbi.nlm.nih.gov/pubmed/15901853 http://dx.doi.org/10.1093/nar/gni081 |