Oligonucleotide-assisted cleavage and ligation: a novel directional DNA cloning technology to capture cDNAs. Application in the construction of a human immune antibody phage-display library

The use of oligonucleotide-assisted cleavage and ligation (ONCL), a novel approach to the capture of gene repertoires, in the construction of a phage-display immune antibody library is described. ONCL begins with rapid amplification of cDNA ends to amplify all members equally. A single, specific cut...

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Detalles Bibliográficos
Autores principales: Schoonbroodt, Sonia, Frans, Nicolas, DeSouza, Mark, Eren, Rachel, Priel, Smadar, Brosh, Naama, Ben-Porath, Judith, Zauberman, Arie, Ilan, Ehud, Dagan, Shlomo, Cohen, Edward H., Hoogenboom, Hennie R., Ladner, Robert Charles, Hoet, René M.
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2005
Materias:
Methods Online
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1131936/
https://www.ncbi.nlm.nih.gov/pubmed/15905471
http://dx.doi.org/10.1093/nar/gni080

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