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Mesenchymally-derived Insulin-like growth factor 1 provides a paracrine stimulus for trophoblast migration
BACKGROUND: Trophoblast migration into maternal decidua is essential for normal pregnancy. It occurs in a defined time window, is spatially highly restricted, and is aberrant in some pathological pregnancies, but the control mechanisms are as yet ill-defined. At the periphery of the placenta, chorio...
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Formato: | Texto |
Lenguaje: | English |
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BioMed Central
2002
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC113269/ https://www.ncbi.nlm.nih.gov/pubmed/11972897 http://dx.doi.org/10.1186/1471-213X-2-5 |
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author | Lacey, Helen Haigh, Teresa Westwood, Melissa Aplin, John D |
author_facet | Lacey, Helen Haigh, Teresa Westwood, Melissa Aplin, John D |
author_sort | Lacey, Helen |
collection | PubMed |
description | BACKGROUND: Trophoblast migration into maternal decidua is essential for normal pregnancy. It occurs in a defined time window, is spatially highly restricted, and is aberrant in some pathological pregnancies, but the control mechanisms are as yet ill-defined. At the periphery of the placenta, chorionic villi make contact with decidua to form specialised anchoring sites that feed interstitially migrating cytotrophoblast into the placental bed. RESULTS: Explants of first trimester mesenchymal villi on collagen type I developed cytotrophoblast outgrowths from the villous tips. However, in medium changed daily, cells did not progress to a migratory phenotype, remaining instead as a contiguous multi-layered sheet. This suggested the need for another migration stimulus. To test the possibility that this might arise from mesenchymal cells, serum-free conditioned medium from first trimester placental fibroblasts was added to explant cultures. Cytotrophoblasts were stimulated to migrate in streams across the gel. Affinity depletion of Insulin-like growth factor from fibroblast medium reduced streaming activity, while the addition of exogenous IGF-I (10 ng/ml) to serum-free medium produced a streaming phenotype. IGF receptor type 1 (IGFR1) was present on cells in the columns, and streaming could be inhibited by antibody to this receptor. IGF-II and activin, known stimulators of cytotrophoblast migration, were also active in this model. CONCLUSIONS: These data suggest a paracrine interaction between villous mesenchyme and the cytotrophoblast in anchoring sites that stimulates trophoblast infiltration of decidua. Such a signal would be self-limiting since it diminishes with distance from the placenta. This is a novel mechanism in placental development. |
format | Text |
id | pubmed-113269 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2002 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-1132692002-05-23 Mesenchymally-derived Insulin-like growth factor 1 provides a paracrine stimulus for trophoblast migration Lacey, Helen Haigh, Teresa Westwood, Melissa Aplin, John D BMC Dev Biol Research Article BACKGROUND: Trophoblast migration into maternal decidua is essential for normal pregnancy. It occurs in a defined time window, is spatially highly restricted, and is aberrant in some pathological pregnancies, but the control mechanisms are as yet ill-defined. At the periphery of the placenta, chorionic villi make contact with decidua to form specialised anchoring sites that feed interstitially migrating cytotrophoblast into the placental bed. RESULTS: Explants of first trimester mesenchymal villi on collagen type I developed cytotrophoblast outgrowths from the villous tips. However, in medium changed daily, cells did not progress to a migratory phenotype, remaining instead as a contiguous multi-layered sheet. This suggested the need for another migration stimulus. To test the possibility that this might arise from mesenchymal cells, serum-free conditioned medium from first trimester placental fibroblasts was added to explant cultures. Cytotrophoblasts were stimulated to migrate in streams across the gel. Affinity depletion of Insulin-like growth factor from fibroblast medium reduced streaming activity, while the addition of exogenous IGF-I (10 ng/ml) to serum-free medium produced a streaming phenotype. IGF receptor type 1 (IGFR1) was present on cells in the columns, and streaming could be inhibited by antibody to this receptor. IGF-II and activin, known stimulators of cytotrophoblast migration, were also active in this model. CONCLUSIONS: These data suggest a paracrine interaction between villous mesenchyme and the cytotrophoblast in anchoring sites that stimulates trophoblast infiltration of decidua. Such a signal would be self-limiting since it diminishes with distance from the placenta. This is a novel mechanism in placental development. BioMed Central 2002-04-24 /pmc/articles/PMC113269/ /pubmed/11972897 http://dx.doi.org/10.1186/1471-213X-2-5 Text en Copyright © 2002 Lacey et al; licensee BioMed Central Ltd. This is an Open Access article: verbatim copying and redistribution of this article are permitted in all media for any purpose, provided this notice is preserved along with the article's original URL. |
spellingShingle | Research Article Lacey, Helen Haigh, Teresa Westwood, Melissa Aplin, John D Mesenchymally-derived Insulin-like growth factor 1 provides a paracrine stimulus for trophoblast migration |
title | Mesenchymally-derived Insulin-like growth factor 1 provides a paracrine stimulus for trophoblast migration |
title_full | Mesenchymally-derived Insulin-like growth factor 1 provides a paracrine stimulus for trophoblast migration |
title_fullStr | Mesenchymally-derived Insulin-like growth factor 1 provides a paracrine stimulus for trophoblast migration |
title_full_unstemmed | Mesenchymally-derived Insulin-like growth factor 1 provides a paracrine stimulus for trophoblast migration |
title_short | Mesenchymally-derived Insulin-like growth factor 1 provides a paracrine stimulus for trophoblast migration |
title_sort | mesenchymally-derived insulin-like growth factor 1 provides a paracrine stimulus for trophoblast migration |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC113269/ https://www.ncbi.nlm.nih.gov/pubmed/11972897 http://dx.doi.org/10.1186/1471-213X-2-5 |
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