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Global analysis of yeast RNA processing identifies new targets of RNase III and uncovers a link between tRNA 5′ end processing and tRNA splicing
We used a microarray containing probes that tile all known yeast noncoding RNAs (ncRNAs) to investigate RNA biogenesis on a global scale. The microarray verified a general loss of Box C/D snoRNAs in the TetO(7)-BCD1 mutant, which had previously been shown for only a handful of snoRNAs. We also monit...
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Formato: | Texto |
Lenguaje: | English |
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Oxford University Press
2005
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1140755/ https://www.ncbi.nlm.nih.gov/pubmed/15920104 http://dx.doi.org/10.1093/nar/gki608 |
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author | Hiley, Shawna L. Babak, Tomas Hughes, Timothy R. |
author_facet | Hiley, Shawna L. Babak, Tomas Hughes, Timothy R. |
author_sort | Hiley, Shawna L. |
collection | PubMed |
description | We used a microarray containing probes that tile all known yeast noncoding RNAs (ncRNAs) to investigate RNA biogenesis on a global scale. The microarray verified a general loss of Box C/D snoRNAs in the TetO(7)-BCD1 mutant, which had previously been shown for only a handful of snoRNAs. We also monitored the accumulation of improperly processed flank sequences of pre-RNAs in strains depleted for known RNA nucleases, including RNase III, Dbr1p, Xrn1p, Rat1p and components of the exosome and RNase P complexes. Among the hundreds of aberrant RNA processing events detected, two novel substrates of Rnt1p (the RUF1 and RUF3 snoRNAs) were identified. We also identified a relationship between tRNA 5′ end processing and tRNA splicing, processes that were previously thought to be independent. This analysis demonstrates the applicability of microarray technology to the study of global analysis of ncRNA synthesis and provides an extensive directory of processing events mediated by yeast ncRNA processing enzymes. |
format | Text |
id | pubmed-1140755 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2005 |
publisher | Oxford University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-11407552005-05-31 Global analysis of yeast RNA processing identifies new targets of RNase III and uncovers a link between tRNA 5′ end processing and tRNA splicing Hiley, Shawna L. Babak, Tomas Hughes, Timothy R. Nucleic Acids Res Article We used a microarray containing probes that tile all known yeast noncoding RNAs (ncRNAs) to investigate RNA biogenesis on a global scale. The microarray verified a general loss of Box C/D snoRNAs in the TetO(7)-BCD1 mutant, which had previously been shown for only a handful of snoRNAs. We also monitored the accumulation of improperly processed flank sequences of pre-RNAs in strains depleted for known RNA nucleases, including RNase III, Dbr1p, Xrn1p, Rat1p and components of the exosome and RNase P complexes. Among the hundreds of aberrant RNA processing events detected, two novel substrates of Rnt1p (the RUF1 and RUF3 snoRNAs) were identified. We also identified a relationship between tRNA 5′ end processing and tRNA splicing, processes that were previously thought to be independent. This analysis demonstrates the applicability of microarray technology to the study of global analysis of ncRNA synthesis and provides an extensive directory of processing events mediated by yeast ncRNA processing enzymes. Oxford University Press 2005 2005-05-26 /pmc/articles/PMC1140755/ /pubmed/15920104 http://dx.doi.org/10.1093/nar/gki608 Text en © The Author 2005. Published by Oxford University Press. All rights reserved |
spellingShingle | Article Hiley, Shawna L. Babak, Tomas Hughes, Timothy R. Global analysis of yeast RNA processing identifies new targets of RNase III and uncovers a link between tRNA 5′ end processing and tRNA splicing |
title | Global analysis of yeast RNA processing identifies new targets of RNase III and uncovers a link between tRNA 5′ end processing and tRNA splicing |
title_full | Global analysis of yeast RNA processing identifies new targets of RNase III and uncovers a link between tRNA 5′ end processing and tRNA splicing |
title_fullStr | Global analysis of yeast RNA processing identifies new targets of RNase III and uncovers a link between tRNA 5′ end processing and tRNA splicing |
title_full_unstemmed | Global analysis of yeast RNA processing identifies new targets of RNase III and uncovers a link between tRNA 5′ end processing and tRNA splicing |
title_short | Global analysis of yeast RNA processing identifies new targets of RNase III and uncovers a link between tRNA 5′ end processing and tRNA splicing |
title_sort | global analysis of yeast rna processing identifies new targets of rnase iii and uncovers a link between trna 5′ end processing and trna splicing |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1140755/ https://www.ncbi.nlm.nih.gov/pubmed/15920104 http://dx.doi.org/10.1093/nar/gki608 |
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