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A real-time semi-quantitative RT–PCR assay demonstrates that the pilE sequence dictates the frequency and characteristics of pilin antigenic variation in Neisseria gonorrhoeae

A semi-quantitative real-time RT–PCR assay was designed to measure gonococcal pilin antigenicvariation (SQ-PCR Av assay). This assay employs 17 hybridization probe sets that quantitate subpopulations of pilin transcripts carrying different silent pilin copy sequences and one set that detects total p...

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Detalles Bibliográficos
Autores principales: Rohrer, Melissa S., Lazio, Matthew P., Seifert, H. Steven
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2005
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1148172/
https://www.ncbi.nlm.nih.gov/pubmed/15947134
http://dx.doi.org/10.1093/nar/gki650