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A real-time semi-quantitative RT–PCR assay demonstrates that the pilE sequence dictates the frequency and characteristics of pilin antigenic variation in Neisseria gonorrhoeae
A semi-quantitative real-time RT–PCR assay was designed to measure gonococcal pilin antigenicvariation (SQ-PCR Av assay). This assay employs 17 hybridization probe sets that quantitate subpopulations of pilin transcripts carrying different silent pilin copy sequences and one set that detects total p...
| Autores principales: | , , |
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| Formato: | Texto |
| Lenguaje: | English |
| Publicado: |
Oxford University Press
2005
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1148172/ https://www.ncbi.nlm.nih.gov/pubmed/15947134 http://dx.doi.org/10.1093/nar/gki650 |