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Selective Permeation and Organic Extraction of Recombinant Green Fluorescent Protein (gfp(uv)) from Escherichia coli

BACKGROUND: Transformed cells of Escherichia coli DH5-α with pGFPuv, induced by IPTG (isopropyl-β-d-thiogalactopyranoside), express the green fluorescent protein (gfp(uv)) during growth phases. E. coli subjected to the combination of selective permeation by freezing/thawing/sonication cycles followe...

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Detalles Bibliográficos
Autores principales:	Christina Vessoni Penna, Thereza, Ishii, Marina
Formato:	Texto
Lenguaje:	English
Publicado:	BioMed Central 2002
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC115201/ https://www.ncbi.nlm.nih.gov/pubmed/11972900 http://dx.doi.org/10.1186/1472-6750-2-7

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