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Low-volume jet injection for intradermal immunization in rabbits

BACKGROUND: This study tested a low-volume (20–30 μl/20–30 μg DNA) jet injection method for intradermal delivery of a DNA vaccine. Jet injection offers the advantages of a needle-less system, low-cost, rapid preparation of the injected DNA solution, and a simple delivery system. More than one constr...

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Detalles Bibliográficos
Autores principales: Ren, Shuxun, Li, Minglin, Smith, Joanne M, DeTolla, Louis J, Furth, Priscilla A
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2002
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC115865/
https://www.ncbi.nlm.nih.gov/pubmed/12028591
http://dx.doi.org/10.1186/1472-6750-2-10
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author Ren, Shuxun
Li, Minglin
Smith, Joanne M
DeTolla, Louis J
Furth, Priscilla A
author_facet Ren, Shuxun
Li, Minglin
Smith, Joanne M
DeTolla, Louis J
Furth, Priscilla A
author_sort Ren, Shuxun
collection PubMed
description BACKGROUND: This study tested a low-volume (20–30 μl/20–30 μg DNA) jet injection method for intradermal delivery of a DNA vaccine. Jet injection offers the advantages of a needle-less system, low-cost, rapid preparation of the injected DNA solution, and a simple delivery system. More than one construct can be injected simultaneously and the method may be combined with adjuvants. RESULTS: Low-volume jet injection targeted delivery of a DNA solution exclusively to the dermis and epidermis of rabbits. A three injection series of plasmid DNA, encoding the Hepatitis B Surface Antigen stimulated a humoral immune response in 2/5 rabbits. One rabbit developed a significant rise in antibody titer after 1 injection and one following 2 injections. There were no significant differences between jet injection and particle bombardment in the maximal antibody titers or number of injections before response. A three injection series of the same plasmid DNA by particle bombardment elicited a significant rise in antibody titer in 3/5 rabbits. One rabbit developed antibody after 1 injection and two after 3 injections. In contrast, 0/5 rabbits receiving DNA by needle and syringe injection responded. In the jet injection and particle bombardment groups, gene expression levels in the skin did not predict response. While immune responses were similar, luciferase gene expression levels in the skin following particle bombardment were 10–100 times higher than jet injection. CONCLUSION: Low-volume jet injection is a simple, effective methodology for intradermal DNA immunization.
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spelling pubmed-1158652002-06-14 Low-volume jet injection for intradermal immunization in rabbits Ren, Shuxun Li, Minglin Smith, Joanne M DeTolla, Louis J Furth, Priscilla A BMC Biotechnol Methodology Article BACKGROUND: This study tested a low-volume (20–30 μl/20–30 μg DNA) jet injection method for intradermal delivery of a DNA vaccine. Jet injection offers the advantages of a needle-less system, low-cost, rapid preparation of the injected DNA solution, and a simple delivery system. More than one construct can be injected simultaneously and the method may be combined with adjuvants. RESULTS: Low-volume jet injection targeted delivery of a DNA solution exclusively to the dermis and epidermis of rabbits. A three injection series of plasmid DNA, encoding the Hepatitis B Surface Antigen stimulated a humoral immune response in 2/5 rabbits. One rabbit developed a significant rise in antibody titer after 1 injection and one following 2 injections. There were no significant differences between jet injection and particle bombardment in the maximal antibody titers or number of injections before response. A three injection series of the same plasmid DNA by particle bombardment elicited a significant rise in antibody titer in 3/5 rabbits. One rabbit developed antibody after 1 injection and two after 3 injections. In contrast, 0/5 rabbits receiving DNA by needle and syringe injection responded. In the jet injection and particle bombardment groups, gene expression levels in the skin did not predict response. While immune responses were similar, luciferase gene expression levels in the skin following particle bombardment were 10–100 times higher than jet injection. CONCLUSION: Low-volume jet injection is a simple, effective methodology for intradermal DNA immunization. BioMed Central 2002-05-23 /pmc/articles/PMC115865/ /pubmed/12028591 http://dx.doi.org/10.1186/1472-6750-2-10 Text en Copyright © 2002 Ren et al; licensee BioMed Central Ltd. This is an Open Access article: verbatim copying and redistribution of this article are permitted in all media for any purpose, provided this notice is preserved along with the article's original URL.
spellingShingle Methodology Article
Ren, Shuxun
Li, Minglin
Smith, Joanne M
DeTolla, Louis J
Furth, Priscilla A
Low-volume jet injection for intradermal immunization in rabbits
title Low-volume jet injection for intradermal immunization in rabbits
title_full Low-volume jet injection for intradermal immunization in rabbits
title_fullStr Low-volume jet injection for intradermal immunization in rabbits
title_full_unstemmed Low-volume jet injection for intradermal immunization in rabbits
title_short Low-volume jet injection for intradermal immunization in rabbits
title_sort low-volume jet injection for intradermal immunization in rabbits
topic Methodology Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC115865/
https://www.ncbi.nlm.nih.gov/pubmed/12028591
http://dx.doi.org/10.1186/1472-6750-2-10
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