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Complete genome sequence of a novel extrachromosomal virus-like element identified in planarian Girardia tigrina

BACKGROUND: Freshwater planarians are widely used as models for investigation of pattern formation and studies on genetic variation in populations. Despite extensive information on the biology and genetics of planaria, the occurrence and distribution of viruses in these animals remains an unexplored...

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Autores principales: Rebrikov, Denis V, Bulina, Maria E, Bogdanova, Ekaterina A, Vagner, Loura L, Lukyanov, Sergey A
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2002
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC116598/
https://www.ncbi.nlm.nih.gov/pubmed/12065025
http://dx.doi.org/10.1186/1471-2164-3-15
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author Rebrikov, Denis V
Bulina, Maria E
Bogdanova, Ekaterina A
Vagner, Loura L
Lukyanov, Sergey A
author_facet Rebrikov, Denis V
Bulina, Maria E
Bogdanova, Ekaterina A
Vagner, Loura L
Lukyanov, Sergey A
author_sort Rebrikov, Denis V
collection PubMed
description BACKGROUND: Freshwater planarians are widely used as models for investigation of pattern formation and studies on genetic variation in populations. Despite extensive information on the biology and genetics of planaria, the occurrence and distribution of viruses in these animals remains an unexplored area of research. RESULTS: Using a combination of Suppression Subtractive Hybridization (SSH) and Mirror Orientation Selection (MOS), we compared the genomes of two strains of freshwater planarian, Girardia tigrina. The novel extrachromosomal DNA-containing virus-like element denoted PEVE (Planarian Extrachromosomal Virus-like Element) was identified in one planarian strain. The PEVE genome (about 7.5 kb) consists of two unique regions (Ul and Us) flanked by inverted repeats. Sequence analyses reveal that PEVE comprises two helicase-like sequences in the genome, of which the first is a homolog of a circoviral replication initiator protein (Rep), and the second is similar to the papillomavirus E1 helicase domain. PEVE genome exists in at least two variant forms with different arrangements of single-stranded and double-stranded DNA stretches that correspond to the Us and Ul regions. Using PCR analysis and whole-mount in situ hybridization, we characterized PEVE distribution and expression in the planarian body. CONCLUSIONS: PEVE is the first viral element identified in free-living flatworms. This element differs from all known viruses and viral elements, and comprises two potential helicases that are homologous to proteins from distant viral phyla. PEVE is unevenly distributed in the worm body, and is detected in specific parenchyma cells.
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spelling pubmed-1165982002-06-28 Complete genome sequence of a novel extrachromosomal virus-like element identified in planarian Girardia tigrina Rebrikov, Denis V Bulina, Maria E Bogdanova, Ekaterina A Vagner, Loura L Lukyanov, Sergey A BMC Genomics Research Article BACKGROUND: Freshwater planarians are widely used as models for investigation of pattern formation and studies on genetic variation in populations. Despite extensive information on the biology and genetics of planaria, the occurrence and distribution of viruses in these animals remains an unexplored area of research. RESULTS: Using a combination of Suppression Subtractive Hybridization (SSH) and Mirror Orientation Selection (MOS), we compared the genomes of two strains of freshwater planarian, Girardia tigrina. The novel extrachromosomal DNA-containing virus-like element denoted PEVE (Planarian Extrachromosomal Virus-like Element) was identified in one planarian strain. The PEVE genome (about 7.5 kb) consists of two unique regions (Ul and Us) flanked by inverted repeats. Sequence analyses reveal that PEVE comprises two helicase-like sequences in the genome, of which the first is a homolog of a circoviral replication initiator protein (Rep), and the second is similar to the papillomavirus E1 helicase domain. PEVE genome exists in at least two variant forms with different arrangements of single-stranded and double-stranded DNA stretches that correspond to the Us and Ul regions. Using PCR analysis and whole-mount in situ hybridization, we characterized PEVE distribution and expression in the planarian body. CONCLUSIONS: PEVE is the first viral element identified in free-living flatworms. This element differs from all known viruses and viral elements, and comprises two potential helicases that are homologous to proteins from distant viral phyla. PEVE is unevenly distributed in the worm body, and is detected in specific parenchyma cells. BioMed Central 2002-06-13 /pmc/articles/PMC116598/ /pubmed/12065025 http://dx.doi.org/10.1186/1471-2164-3-15 Text en Copyright © 2002 Rebrikov et al; licensee BioMed Central Ltd. This is an Open Access article: verbatim copying and redistribution of this article are permitted in all media for any purpose, provided this notice is preserved along with the article's original URL.
spellingShingle Research Article
Rebrikov, Denis V
Bulina, Maria E
Bogdanova, Ekaterina A
Vagner, Loura L
Lukyanov, Sergey A
Complete genome sequence of a novel extrachromosomal virus-like element identified in planarian Girardia tigrina
title Complete genome sequence of a novel extrachromosomal virus-like element identified in planarian Girardia tigrina
title_full Complete genome sequence of a novel extrachromosomal virus-like element identified in planarian Girardia tigrina
title_fullStr Complete genome sequence of a novel extrachromosomal virus-like element identified in planarian Girardia tigrina
title_full_unstemmed Complete genome sequence of a novel extrachromosomal virus-like element identified in planarian Girardia tigrina
title_short Complete genome sequence of a novel extrachromosomal virus-like element identified in planarian Girardia tigrina
title_sort complete genome sequence of a novel extrachromosomal virus-like element identified in planarian girardia tigrina
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC116598/
https://www.ncbi.nlm.nih.gov/pubmed/12065025
http://dx.doi.org/10.1186/1471-2164-3-15
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