Identification of Cellular Membrane Proteins Interacting with Hepatitis B Surface Antigen using Yeast Split-Ubiquitin System

Hepatitis B surface antigen (HBsAg) is the major component of the envelope of hepatitis B virus (HBV). As a resident membrane protein in the endoplasmic reticulum, it plays a key role in the viral morphogenesis. Little is known about cellular proteins that interact with HBsAg and thereby contributin...

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Autores principales: Toh, Qi Chun, Tan, Tuan Lin, Teo, Wei Qiang, Ho, Chin Yee, Parida, Subhajeet, Chen, Wei Ning
Formato: Texto
Lenguaje:English
Publicado: Ivyspring International Publisher 2005
Materias:
Short Research Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1168876/
https://www.ncbi.nlm.nih.gov/pubmed/16007263
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author Toh, Qi Chun
Tan, Tuan Lin
Teo, Wei Qiang
Ho, Chin Yee
Parida, Subhajeet
Chen, Wei Ning
author_facet Toh, Qi Chun
Tan, Tuan Lin
Teo, Wei Qiang
Ho, Chin Yee
Parida, Subhajeet
Chen, Wei Ning
author_sort Toh, Qi Chun
collection PubMed
description Hepatitis B surface antigen (HBsAg) is the major component of the envelope of hepatitis B virus (HBV). As a resident membrane protein in the endoplasmic reticulum, it plays a key role in the viral morphogenesis. Little is known about cellular proteins that interact with HBsAg and thereby contributing to HBV morphogenesis. Using the yeast split-ubiquitin system, a number of cellular membrane proteins have been isolated in this study. These include a resident protein of endoplasmic reticulum (thioredoxin-related transmembrane protein 2), an adaptor protein involved in clathrin-mediated endocytosis and HIV-mediated downregulation of CD4, and a co-receptor of coxsakie B virus. The significance of our findings is suggested by the identification of cellular membrane proteins interacting with other virus proteins. Further functional analysis of these HBsAg- interacting cellular membrane proteins should shed new insights on their role in HBV morphogenesis.
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spelling pubmed-11688762005-07-07 Identification of Cellular Membrane Proteins Interacting with Hepatitis B Surface Antigen using Yeast Split-Ubiquitin System Toh, Qi Chun Tan, Tuan Lin Teo, Wei Qiang Ho, Chin Yee Parida, Subhajeet Chen, Wei Ning Int J Med Sci Short Research Communication Hepatitis B surface antigen (HBsAg) is the major component of the envelope of hepatitis B virus (HBV). As a resident membrane protein in the endoplasmic reticulum, it plays a key role in the viral morphogenesis. Little is known about cellular proteins that interact with HBsAg and thereby contributing to HBV morphogenesis. Using the yeast split-ubiquitin system, a number of cellular membrane proteins have been isolated in this study. These include a resident protein of endoplasmic reticulum (thioredoxin-related transmembrane protein 2), an adaptor protein involved in clathrin-mediated endocytosis and HIV-mediated downregulation of CD4, and a co-receptor of coxsakie B virus. The significance of our findings is suggested by the identification of cellular membrane proteins interacting with other virus proteins. Further functional analysis of these HBsAg- interacting cellular membrane proteins should shed new insights on their role in HBV morphogenesis. Ivyspring International Publisher 2005-07-05 /pmc/articles/PMC1168876/ /pubmed/16007263 Text en © Ivyspring International Publisher. This is an open access article distributed under the terms of a Creative Commons License which permits distribution and reproduction for noncommerical purposes, provided that the article is in whole, unmodified, and properly cited.
spellingShingle Short Research Communication
Toh, Qi Chun
Tan, Tuan Lin
Teo, Wei Qiang
Ho, Chin Yee
Parida, Subhajeet
Chen, Wei Ning
Identification of Cellular Membrane Proteins Interacting with Hepatitis B Surface Antigen using Yeast Split-Ubiquitin System
title Identification of Cellular Membrane Proteins Interacting with Hepatitis B Surface Antigen using Yeast Split-Ubiquitin System
title_full Identification of Cellular Membrane Proteins Interacting with Hepatitis B Surface Antigen using Yeast Split-Ubiquitin System
title_fullStr Identification of Cellular Membrane Proteins Interacting with Hepatitis B Surface Antigen using Yeast Split-Ubiquitin System
title_full_unstemmed Identification of Cellular Membrane Proteins Interacting with Hepatitis B Surface Antigen using Yeast Split-Ubiquitin System
title_short Identification of Cellular Membrane Proteins Interacting with Hepatitis B Surface Antigen using Yeast Split-Ubiquitin System
title_sort identification of cellular membrane proteins interacting with hepatitis b surface antigen using yeast split-ubiquitin system
topic Short Research Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1168876/
https://www.ncbi.nlm.nih.gov/pubmed/16007263
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