A new way to rapidly create functional, fluorescent fusion proteins: random insertion of GFP with an in vitro transposition reaction

BACKGROUND: The jellyfish green fluorescent protein (GFP) can be inserted into the middle of another protein to produce a functional, fluorescent fusion protein. Finding permissive sites for insertion, however, can be difficult. Here we describe a transposon-based approach for rapidly creating libra...

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Detalles Bibliográficos
Autores principales: Sheridan, Douglas L, Berlot, Catherine H, Robert, Antoine, Inglis, Fiona M, Jakobsdottir, Klara B, Howe, James R, Hughes, Thomas E
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2002
Materias:
Methodology Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC117241/
https://www.ncbi.nlm.nih.gov/pubmed/12086589
http://dx.doi.org/10.1186/1471-2202-3-7

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