Cargando…

A mutation in the centriole-associated protein centrin causes genomic instability via increased chromosome loss in Chlamydomonas reinhardtii

BACKGROUND: The role of centrioles in mitotic spindle function remains unclear. One approach to investigate mitotic centriole function is to ask whether mutation of centriole-associated proteins can cause genomic instability. RESULTS: We addressed the role of the centriole-associated EF-hand protein...

Descripción completa

Detalles Bibliográficos
Autores principales: Zamora, Ivan, Marshall, Wallace F
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2005
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1174865/
https://www.ncbi.nlm.nih.gov/pubmed/15927066
http://dx.doi.org/10.1186/1741-7007-3-15
_version_ 1782124469059321856
author Zamora, Ivan
Marshall, Wallace F
author_facet Zamora, Ivan
Marshall, Wallace F
author_sort Zamora, Ivan
collection PubMed
description BACKGROUND: The role of centrioles in mitotic spindle function remains unclear. One approach to investigate mitotic centriole function is to ask whether mutation of centriole-associated proteins can cause genomic instability. RESULTS: We addressed the role of the centriole-associated EF-hand protein centrin in genomic stability using a Chlamydomonas reinhardtii centrin mutant that forms acentriolar bipolar spindles and lacks the centrin-based rhizoplast structures that join centrioles to the nucleus. Using a genetic assay for loss of heterozygosity, we found that this centrin mutant showed increased genomic instability compared to wild-type cells, and we determined that the increase in genomic instability was due to a 100-fold increase in chromosome loss rates compared to wild type. Live cell imaging reveals an increased rate in cell death during G1 in haploid cells that is consistent with an elevated rate of chromosome loss, and analysis of cell death versus centriole copy number argues against a role for multipolar spindles in this process. CONCLUSION: The increased chromosome loss rates observed in a centrin mutant that forms acentriolar spindles suggests a role for centrin protein, and possibly centrioles, in mitotic fidelity.
format Text
id pubmed-1174865
institution National Center for Biotechnology Information
language English
publishDate 2005
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-11748652005-07-09 A mutation in the centriole-associated protein centrin causes genomic instability via increased chromosome loss in Chlamydomonas reinhardtii Zamora, Ivan Marshall, Wallace F BMC Biol Research Article BACKGROUND: The role of centrioles in mitotic spindle function remains unclear. One approach to investigate mitotic centriole function is to ask whether mutation of centriole-associated proteins can cause genomic instability. RESULTS: We addressed the role of the centriole-associated EF-hand protein centrin in genomic stability using a Chlamydomonas reinhardtii centrin mutant that forms acentriolar bipolar spindles and lacks the centrin-based rhizoplast structures that join centrioles to the nucleus. Using a genetic assay for loss of heterozygosity, we found that this centrin mutant showed increased genomic instability compared to wild-type cells, and we determined that the increase in genomic instability was due to a 100-fold increase in chromosome loss rates compared to wild type. Live cell imaging reveals an increased rate in cell death during G1 in haploid cells that is consistent with an elevated rate of chromosome loss, and analysis of cell death versus centriole copy number argues against a role for multipolar spindles in this process. CONCLUSION: The increased chromosome loss rates observed in a centrin mutant that forms acentriolar spindles suggests a role for centrin protein, and possibly centrioles, in mitotic fidelity. BioMed Central 2005-05-31 /pmc/articles/PMC1174865/ /pubmed/15927066 http://dx.doi.org/10.1186/1741-7007-3-15 Text en Copyright © 2005 Zamora and Marshall; licensee BioMed Central Ltd.
spellingShingle Research Article
Zamora, Ivan
Marshall, Wallace F
A mutation in the centriole-associated protein centrin causes genomic instability via increased chromosome loss in Chlamydomonas reinhardtii
title A mutation in the centriole-associated protein centrin causes genomic instability via increased chromosome loss in Chlamydomonas reinhardtii
title_full A mutation in the centriole-associated protein centrin causes genomic instability via increased chromosome loss in Chlamydomonas reinhardtii
title_fullStr A mutation in the centriole-associated protein centrin causes genomic instability via increased chromosome loss in Chlamydomonas reinhardtii
title_full_unstemmed A mutation in the centriole-associated protein centrin causes genomic instability via increased chromosome loss in Chlamydomonas reinhardtii
title_short A mutation in the centriole-associated protein centrin causes genomic instability via increased chromosome loss in Chlamydomonas reinhardtii
title_sort mutation in the centriole-associated protein centrin causes genomic instability via increased chromosome loss in chlamydomonas reinhardtii
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1174865/
https://www.ncbi.nlm.nih.gov/pubmed/15927066
http://dx.doi.org/10.1186/1741-7007-3-15
work_keys_str_mv AT zamoraivan amutationinthecentrioleassociatedproteincentrincausesgenomicinstabilityviaincreasedchromosomelossinchlamydomonasreinhardtii
AT marshallwallacef amutationinthecentrioleassociatedproteincentrincausesgenomicinstabilityviaincreasedchromosomelossinchlamydomonasreinhardtii
AT zamoraivan mutationinthecentrioleassociatedproteincentrincausesgenomicinstabilityviaincreasedchromosomelossinchlamydomonasreinhardtii
AT marshallwallacef mutationinthecentrioleassociatedproteincentrincausesgenomicinstabilityviaincreasedchromosomelossinchlamydomonasreinhardtii