A novel high-throughput (HTP) cloning strategy for site-directed designed chimeragenesis and mutation using the Gateway cloning system

There is an increasing demand for easy, high-throughput (HTP) methods for protein engineering to support advances in the development of structural biology, bioinformatics and drug design. Here, we describe an N- and C-terminal cloning method utilizing Gateway cloning technology that we have adopted...

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Detalles Bibliográficos
Autores principales: Suzuki, Yasuhiro, Kagawa, Naoko, Fujino, Toru, Sumiya, Tsuyoshi, Andoh, Taichi, Ishikawa, Kumiko, Kimura, Rie, Kemmochi, Kiyokazu, Ohta, Tsutomu, Tanaka, Shigeo
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2005
Materias:
Methods Online
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1174934/
https://www.ncbi.nlm.nih.gov/pubmed/16009811
http://dx.doi.org/10.1093/nar/gni103

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1174934/
https://www.ncbi.nlm.nih.gov/pubmed/16009811
http://dx.doi.org/10.1093/nar/gni103

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