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Gene expression profile and synovial microcirculation at early stages of collagen-induced arthritis

A better understanding of the initial mechanisms that lead to arthritic disease could facilitate development of improved therapeutic strategies. We characterized the synovial microcirculation of knee joints in susceptible mouse strains undergoing intradermal immunization with bovine collagen II in c...

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Autores principales: Gierer, Philip, Ibrahim, Saleh, Mittlmeier, Thomas, Koczan, Dirk, Moeller, Steffen, Landes, Jürgen, Gradl, Georg, Vollmar, Brigitte
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2005
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1175036/
https://www.ncbi.nlm.nih.gov/pubmed/15987489
http://dx.doi.org/10.1186/ar1754
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author Gierer, Philip
Ibrahim, Saleh
Mittlmeier, Thomas
Koczan, Dirk
Moeller, Steffen
Landes, Jürgen
Gradl, Georg
Vollmar, Brigitte
author_facet Gierer, Philip
Ibrahim, Saleh
Mittlmeier, Thomas
Koczan, Dirk
Moeller, Steffen
Landes, Jürgen
Gradl, Georg
Vollmar, Brigitte
author_sort Gierer, Philip
collection PubMed
description A better understanding of the initial mechanisms that lead to arthritic disease could facilitate development of improved therapeutic strategies. We characterized the synovial microcirculation of knee joints in susceptible mouse strains undergoing intradermal immunization with bovine collagen II in complete Freund's adjuvant to induce arthritis (i.e. collagen-induced arthritis [CIA]). Susceptible DBA1/J and collagen II T-cell receptor transgenic mice were compared with CIA-resistant FVB/NJ mice. Before onset of clinical symptoms of arthritis, in vivo fluorescence microscopy of knee joints revealed marked leucocyte activation and interaction with the endothelial lining of synovial microvessels. This initial inflammatory cell response correlated with the gene expression profile at this disease stage. The majority of the 655 differentially expressed genes belonged to classes of genes that are involved in cell movement and structure, cell cycle and signal transduction, as well as transcription, protein synthesis and metabolism. However, 24 adhesion molecules and chemokine/cytokine genes were identified, some of which are known to contribute to arthritis (e.g. CD44 and neutrophil cytosolic factor 1) and some of which are novel in this respect (e.g. CC chemokine ligand-27 and IL-13 receptor α(1)). Online in vivo data on synovial tissue microcirculation, together with gene expression profiling, emphasize the potential role played by early inflammatory events in the development of arthritis.
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spelling pubmed-11750362005-07-14 Gene expression profile and synovial microcirculation at early stages of collagen-induced arthritis Gierer, Philip Ibrahim, Saleh Mittlmeier, Thomas Koczan, Dirk Moeller, Steffen Landes, Jürgen Gradl, Georg Vollmar, Brigitte Arthritis Res Ther Research Article A better understanding of the initial mechanisms that lead to arthritic disease could facilitate development of improved therapeutic strategies. We characterized the synovial microcirculation of knee joints in susceptible mouse strains undergoing intradermal immunization with bovine collagen II in complete Freund's adjuvant to induce arthritis (i.e. collagen-induced arthritis [CIA]). Susceptible DBA1/J and collagen II T-cell receptor transgenic mice were compared with CIA-resistant FVB/NJ mice. Before onset of clinical symptoms of arthritis, in vivo fluorescence microscopy of knee joints revealed marked leucocyte activation and interaction with the endothelial lining of synovial microvessels. This initial inflammatory cell response correlated with the gene expression profile at this disease stage. The majority of the 655 differentially expressed genes belonged to classes of genes that are involved in cell movement and structure, cell cycle and signal transduction, as well as transcription, protein synthesis and metabolism. However, 24 adhesion molecules and chemokine/cytokine genes were identified, some of which are known to contribute to arthritis (e.g. CD44 and neutrophil cytosolic factor 1) and some of which are novel in this respect (e.g. CC chemokine ligand-27 and IL-13 receptor α(1)). Online in vivo data on synovial tissue microcirculation, together with gene expression profiling, emphasize the potential role played by early inflammatory events in the development of arthritis. BioMed Central 2005 2005-05-17 /pmc/articles/PMC1175036/ /pubmed/15987489 http://dx.doi.org/10.1186/ar1754 Text en Copyright © 2005 Gierer et al.; licensee BioMed Central Ltd.
spellingShingle Research Article
Gierer, Philip
Ibrahim, Saleh
Mittlmeier, Thomas
Koczan, Dirk
Moeller, Steffen
Landes, Jürgen
Gradl, Georg
Vollmar, Brigitte
Gene expression profile and synovial microcirculation at early stages of collagen-induced arthritis
title Gene expression profile and synovial microcirculation at early stages of collagen-induced arthritis
title_full Gene expression profile and synovial microcirculation at early stages of collagen-induced arthritis
title_fullStr Gene expression profile and synovial microcirculation at early stages of collagen-induced arthritis
title_full_unstemmed Gene expression profile and synovial microcirculation at early stages of collagen-induced arthritis
title_short Gene expression profile and synovial microcirculation at early stages of collagen-induced arthritis
title_sort gene expression profile and synovial microcirculation at early stages of collagen-induced arthritis
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1175036/
https://www.ncbi.nlm.nih.gov/pubmed/15987489
http://dx.doi.org/10.1186/ar1754
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