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Characterization of the aggregates formed during recombinant protein expression in bacteria
BACKGROUND: The first aim of the work was to analyze in detail the complexity of the aggregates formed upon overexpression of recombinant proteins in E. coli. A sucrose step gradient succeeded in separating aggregate subclasses of a GFP-GST fusion protein with specific biochemical and biophysical fe...
| Autores principales: | , |
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| Formato: | Texto |
| Lenguaje: | English |
| Publicado: |
BioMed Central
2005
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1175841/ https://www.ncbi.nlm.nih.gov/pubmed/15927061 http://dx.doi.org/10.1186/1471-2091-6-10 |