Genomic analysis of heat-shock factor targets in Drosophila

We have used a chromatin immunoprecipitation-microarray (ChIP-array) approach to investigate the in vivo targets of heat-shock factor (Hsf) in Drosophila embryos. We show that this method identifies Hsf target sites with high fidelity and resolution. Using cDNA arrays in a genomic search for Hsf tar...

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Detalles Bibliográficos
Autores principales: Birch-Machin, Ian, Gao, Shan, Huen, David, McGirr, Richard, White, Robert AH, Russell, Steven
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2005
Materias:
Method
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1175994/
https://www.ncbi.nlm.nih.gov/pubmed/15998452
http://dx.doi.org/10.1186/gb-2005-6-7-r63
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author Birch-Machin, Ian
Gao, Shan
Huen, David
McGirr, Richard
White, Robert AH
Russell, Steven
author_facet Birch-Machin, Ian
Gao, Shan
Huen, David
McGirr, Richard
White, Robert AH
Russell, Steven
author_sort Birch-Machin, Ian
collection PubMed
description We have used a chromatin immunoprecipitation-microarray (ChIP-array) approach to investigate the in vivo targets of heat-shock factor (Hsf) in Drosophila embryos. We show that this method identifies Hsf target sites with high fidelity and resolution. Using cDNA arrays in a genomic search for Hsf targets, we identified 141 genes with highly significant ChIP enrichment. This study firmly establishes the potential of ChIP-array for whole-genome transcription factor target mapping in vivo using intact whole organisms.
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spelling pubmed-11759942005-07-17 Genomic analysis of heat-shock factor targets in Drosophila Birch-Machin, Ian Gao, Shan Huen, David McGirr, Richard White, Robert AH Russell, Steven Genome Biol Method We have used a chromatin immunoprecipitation-microarray (ChIP-array) approach to investigate the in vivo targets of heat-shock factor (Hsf) in Drosophila embryos. We show that this method identifies Hsf target sites with high fidelity and resolution. Using cDNA arrays in a genomic search for Hsf targets, we identified 141 genes with highly significant ChIP enrichment. This study firmly establishes the potential of ChIP-array for whole-genome transcription factor target mapping in vivo using intact whole organisms. BioMed Central 2005 2005-06-10 /pmc/articles/PMC1175994/ /pubmed/15998452 http://dx.doi.org/10.1186/gb-2005-6-7-r63 Text en Copyright © 2005 Birch-Machin et al.; licensee BioMed Central Ltd.
spellingShingle Method
Birch-Machin, Ian
Gao, Shan
Huen, David
McGirr, Richard
White, Robert AH
Russell, Steven
Genomic analysis of heat-shock factor targets in Drosophila
title Genomic analysis of heat-shock factor targets in Drosophila
title_full Genomic analysis of heat-shock factor targets in Drosophila
title_fullStr Genomic analysis of heat-shock factor targets in Drosophila
title_full_unstemmed Genomic analysis of heat-shock factor targets in Drosophila
title_short Genomic analysis of heat-shock factor targets in Drosophila
title_sort genomic analysis of heat-shock factor targets in drosophila
topic Method
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1175994/
https://www.ncbi.nlm.nih.gov/pubmed/15998452
http://dx.doi.org/10.1186/gb-2005-6-7-r63
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