A novel PCR strategy for high-efficiency, automated site-directed mutagenesis

We have developed a novel three-primer, one-step PCR-based method for site-directed mutagenesis. This method takes advantage of the fact that template plasmid DNA cannot be efficiently denatured at its reannealing temperature (T(ra)), which is otherwise a troublesome problem in regular PCR. Two flan...

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Detalles Bibliográficos
Autores principales: Wu, Wu, Jia, Zongchao, Liu, Ping, Xie, Zhigang, Wei, Qun
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2005
Materias:
Methods Online
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1178011/
https://www.ncbi.nlm.nih.gov/pubmed/16030347
http://dx.doi.org/10.1093/nar/gni115

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1178011/
https://www.ncbi.nlm.nih.gov/pubmed/16030347
http://dx.doi.org/10.1093/nar/gni115

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