Nucleotide exchange and excision technology (NExT) DNA shuffling: a robust method for DNA fragmentation and directed evolution

DNA shuffling is widely used for optimizing complex properties contained within DNA and proteins. Demonstrated here is the amplification of a gene library by PCR using uridine triphosphate (dUTP) as a fragmentation defining exchange nucleotide with thymidine, together with the three other nucleotide...

Descripción completa

Detalles Bibliográficos
Autores principales: Müller, Kristian M., Stebel, Sabine C., Knall, Susanne, Zipf, Gregor, Bernauer, Hubert S., Arndt, Katja M.
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2005
Materias:
Methods Online
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1182171/
https://www.ncbi.nlm.nih.gov/pubmed/16061932
http://dx.doi.org/10.1093/nar/gni116

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1182171/
https://www.ncbi.nlm.nih.gov/pubmed/16061932
http://dx.doi.org/10.1093/nar/gni116

Ejemplares similares