Cargando…
SiteFinding-PCR: a simple and efficient PCR method for chromosome walking
In this paper, we present a novel PCR method, termed SiteFinding-PCR, for gene or chromosome walking. The PCR was primed by a SiteFinder at a low temperature, and then the target molecules were amplified exponentially with gene-specific and SiteFinder primers, and screened out by another gene-specif...
Autores principales: | Tan, Guihong, Gao, Yin, Shi, Miao, Zhang, Xinyue, He, Shanping, Chen, Zhangliang, An, Chengcai |
---|---|
Formato: | Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2005
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1182332/ https://www.ncbi.nlm.nih.gov/pubmed/16077029 http://dx.doi.org/10.1093/nar/gni124 |
Ejemplares similares
-
A novel PCR strategy for high-efficiency, automated site-directed mutagenesis
por: Wu, Wu, et al.
Publicado: (2005) -
Enhanced analysis of real-time PCR data by using a variable efficiency model: FPK-PCR
por: Lievens, Antoon, et al.
Publicado: (2012) -
Accurate quantification of DNA using on-site PCR (osPCR) by characterizing DNA amplification at single-molecule resolution
por: Ding, Ruihua, et al.
Publicado: (2023) -
Enhancing the efficiency of a PCR using gold nanoparticles
por: Li, Min, et al.
Publicado: (2005) -
PCR inhibition by reverse transcriptase leads to an overestimation of amplification efficiency
por: Suslov, Oleg, et al.
Publicado: (2005)