Cargando…

Serum nucleosomes during neoadjuvant chemotherapy in patients with cervical cancer. Predictive and prognostic significance

BACKGROUND: It has been shown that free DNA circulates in serum plasma of patients with cancer and that at least part is present in the form of oligo- and monucleosomes, a marker of cell death. Preliminary data has shown a good correlation between decrease of nucleosomes with response and prognosis....

Descripción completa

Detalles Bibliográficos
Autores principales: Trejo-Becerril, Catalina, Oñate-Ocaña, Luis F, Taja-Chayeb, Lucía, Vanoye-Carlo, América, Cetina, Lucely, Duenas-Gonzalez, Alfonso
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2005
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1182357/
https://www.ncbi.nlm.nih.gov/pubmed/15982417
http://dx.doi.org/10.1186/1471-2407-5-65
_version_ 1782124658732040192
author Trejo-Becerril, Catalina
Oñate-Ocaña, Luis F
Taja-Chayeb, Lucía
Vanoye-Carlo, América
Cetina, Lucely
Duenas-Gonzalez, Alfonso
author_facet Trejo-Becerril, Catalina
Oñate-Ocaña, Luis F
Taja-Chayeb, Lucía
Vanoye-Carlo, América
Cetina, Lucely
Duenas-Gonzalez, Alfonso
author_sort Trejo-Becerril, Catalina
collection PubMed
description BACKGROUND: It has been shown that free DNA circulates in serum plasma of patients with cancer and that at least part is present in the form of oligo- and monucleosomes, a marker of cell death. Preliminary data has shown a good correlation between decrease of nucleosomes with response and prognosis. Here, we performed pre- and post-chemotherapy determinations of serum nucleosomes with an enzyme-linked immunosorbent assay (ELISA) method in a group of patients with cervical cancer receiving neoadjuvant chemotherapy. METHODS: From December 2000 to June 2001, 41 patients with cervical cancer staged as FIGO stages IB2-IIIB received three 21-day courses of carboplatin and paclitaxel, both administered at day 1; then, patients underwent radical hysterectomy. Nucleosomes were measured the day before (baseline), at day seven of the first course and day seven of the third course of chemotherapy. Values of nucleosomes were analyzed with regard to pathologic response and to time to progression-free and overall survival. RESULTS: All patients completed chemotherapy, were evaluable for pathologic response, and had nucleosome levels determined. At a mean follow-up of 23 months (range, 7–26 months), projected progression time and overall survival were 80.3 and 80.4%, respectively. Mean differential values of nucleosomes were lower in the third course as compared with the first course (p >0.001). The decrease in the third course correlated with pathologic response (p = 0.041). Survival analysis showed a statistically significant, better progression-free and survival time in patients who showed lower levels at the third course (p = 0.0243 and p = 0.0260, respectively). Cox regression analysis demonstrated that nucleosome increase in the third course increased risk of death to 6.86 (95% confidence interval [CI 95%], 0.84–56.0). CONCLUSION: Serum nucleosomes may have a predictive role for response and prognostic significance in patients with cervical cancer patients treated with neoadjuvant chemotherapy.
format Text
id pubmed-1182357
institution National Center for Biotechnology Information
language English
publishDate 2005
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-11823572005-08-04 Serum nucleosomes during neoadjuvant chemotherapy in patients with cervical cancer. Predictive and prognostic significance Trejo-Becerril, Catalina Oñate-Ocaña, Luis F Taja-Chayeb, Lucía Vanoye-Carlo, América Cetina, Lucely Duenas-Gonzalez, Alfonso BMC Cancer Research Article BACKGROUND: It has been shown that free DNA circulates in serum plasma of patients with cancer and that at least part is present in the form of oligo- and monucleosomes, a marker of cell death. Preliminary data has shown a good correlation between decrease of nucleosomes with response and prognosis. Here, we performed pre- and post-chemotherapy determinations of serum nucleosomes with an enzyme-linked immunosorbent assay (ELISA) method in a group of patients with cervical cancer receiving neoadjuvant chemotherapy. METHODS: From December 2000 to June 2001, 41 patients with cervical cancer staged as FIGO stages IB2-IIIB received three 21-day courses of carboplatin and paclitaxel, both administered at day 1; then, patients underwent radical hysterectomy. Nucleosomes were measured the day before (baseline), at day seven of the first course and day seven of the third course of chemotherapy. Values of nucleosomes were analyzed with regard to pathologic response and to time to progression-free and overall survival. RESULTS: All patients completed chemotherapy, were evaluable for pathologic response, and had nucleosome levels determined. At a mean follow-up of 23 months (range, 7–26 months), projected progression time and overall survival were 80.3 and 80.4%, respectively. Mean differential values of nucleosomes were lower in the third course as compared with the first course (p >0.001). The decrease in the third course correlated with pathologic response (p = 0.041). Survival analysis showed a statistically significant, better progression-free and survival time in patients who showed lower levels at the third course (p = 0.0243 and p = 0.0260, respectively). Cox regression analysis demonstrated that nucleosome increase in the third course increased risk of death to 6.86 (95% confidence interval [CI 95%], 0.84–56.0). CONCLUSION: Serum nucleosomes may have a predictive role for response and prognostic significance in patients with cervical cancer patients treated with neoadjuvant chemotherapy. BioMed Central 2005-06-27 /pmc/articles/PMC1182357/ /pubmed/15982417 http://dx.doi.org/10.1186/1471-2407-5-65 Text en Copyright © 2005 Trejo-Becerril et al; licensee BioMed Central Ltd.
spellingShingle Research Article
Trejo-Becerril, Catalina
Oñate-Ocaña, Luis F
Taja-Chayeb, Lucía
Vanoye-Carlo, América
Cetina, Lucely
Duenas-Gonzalez, Alfonso
Serum nucleosomes during neoadjuvant chemotherapy in patients with cervical cancer. Predictive and prognostic significance
title Serum nucleosomes during neoadjuvant chemotherapy in patients with cervical cancer. Predictive and prognostic significance
title_full Serum nucleosomes during neoadjuvant chemotherapy in patients with cervical cancer. Predictive and prognostic significance
title_fullStr Serum nucleosomes during neoadjuvant chemotherapy in patients with cervical cancer. Predictive and prognostic significance
title_full_unstemmed Serum nucleosomes during neoadjuvant chemotherapy in patients with cervical cancer. Predictive and prognostic significance
title_short Serum nucleosomes during neoadjuvant chemotherapy in patients with cervical cancer. Predictive and prognostic significance
title_sort serum nucleosomes during neoadjuvant chemotherapy in patients with cervical cancer. predictive and prognostic significance
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1182357/
https://www.ncbi.nlm.nih.gov/pubmed/15982417
http://dx.doi.org/10.1186/1471-2407-5-65
work_keys_str_mv AT trejobecerrilcatalina serumnucleosomesduringneoadjuvantchemotherapyinpatientswithcervicalcancerpredictiveandprognosticsignificance
AT onateocanaluisf serumnucleosomesduringneoadjuvantchemotherapyinpatientswithcervicalcancerpredictiveandprognosticsignificance
AT tajachayeblucia serumnucleosomesduringneoadjuvantchemotherapyinpatientswithcervicalcancerpredictiveandprognosticsignificance
AT vanoyecarloamerica serumnucleosomesduringneoadjuvantchemotherapyinpatientswithcervicalcancerpredictiveandprognosticsignificance
AT cetinalucely serumnucleosomesduringneoadjuvantchemotherapyinpatientswithcervicalcancerpredictiveandprognosticsignificance
AT duenasgonzalezalfonso serumnucleosomesduringneoadjuvantchemotherapyinpatientswithcervicalcancerpredictiveandprognosticsignificance