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Enhanced detergent extraction for analysis of membrane proteomes by two-dimensional gel electrophoresis

BACKGROUND: The analysis of hydrophobic membrane proteins by two-dimensional gel electrophoresis has long been hampered by the concept of inherent difficulty due to solubility issues. We have optimized extraction protocols by varying the detergent composition of the solubilization buffer with a vari...

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Detalles Bibliográficos
Autores principales:	Churchward, Matthew A, Butt, R Hussain, Lang, John C, Hsu, Kimberly K, Coorssen, Jens R
Formato:	Texto
Lenguaje:	English
Publicado:	BioMed Central 2005
Materias:	Methodology
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1184097/ https://www.ncbi.nlm.nih.gov/pubmed/15941475 http://dx.doi.org/10.1186/1477-5956-3-5

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