Rapid cryopreservation of five mammalian and one mosquito cell line at -80°C while attached to flasks in a serum free cryopreservative

Cell culturing, and the requisite storage of cell lines at ultra-low temperatures, is used in most laboratories studying or using eukaryotic proteomics, genomics, microarray, and RNA technologies. In this study we have observed that A72(dog), CRFK(cat), NB324K(human), MCF7(human), WI38(human), and C...

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Detalles Bibliográficos
Autores principales: Corsini, Joe, Mann, Ethan
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2005
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1190376/
https://www.ncbi.nlm.nih.gov/pubmed/16136221
http://dx.doi.org/10.1251/bpo102
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author Corsini, Joe
Mann, Ethan
author_facet Corsini, Joe
Mann, Ethan
author_sort Corsini, Joe
collection PubMed
description Cell culturing, and the requisite storage of cell lines at ultra-low temperatures, is used in most laboratories studying or using eukaryotic proteomics, genomics, microarray, and RNA technologies. In this study we have observed that A72(dog), CRFK(cat), NB324K(human), MCF7(human), WI38(human), and C636(mosquito) cells were effectively cryopreserved at -80°C while attached to the substratum of 25cm(2 )tissue culture flasks. This was accomplished using a serum free crypreservative recently developed by Corsini and co-workers. The technique allows for significant savings of time and money in laboratories that rapidly process numerous cell lines.
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spelling pubmed-11903762005-08-31 Rapid cryopreservation of five mammalian and one mosquito cell line at -80°C while attached to flasks in a serum free cryopreservative Corsini, Joe Mann, Ethan Biol Proced Online Research Article Cell culturing, and the requisite storage of cell lines at ultra-low temperatures, is used in most laboratories studying or using eukaryotic proteomics, genomics, microarray, and RNA technologies. In this study we have observed that A72(dog), CRFK(cat), NB324K(human), MCF7(human), WI38(human), and C636(mosquito) cells were effectively cryopreserved at -80°C while attached to the substratum of 25cm(2 )tissue culture flasks. This was accomplished using a serum free crypreservative recently developed by Corsini and co-workers. The technique allows for significant savings of time and money in laboratories that rapidly process numerous cell lines. BioMed Central 2005-03-14 /pmc/articles/PMC1190376/ /pubmed/16136221 http://dx.doi.org/10.1251/bpo102 Text en Copyright © March 03, 2005, J Corsini et al. This paper is Open Access and is published in Biological Procedures Online under license from the authors. Copying, printing, redistribution and storage permitted.
spellingShingle Research Article
Corsini, Joe
Mann, Ethan
Rapid cryopreservation of five mammalian and one mosquito cell line at -80°C while attached to flasks in a serum free cryopreservative
title Rapid cryopreservation of five mammalian and one mosquito cell line at -80°C while attached to flasks in a serum free cryopreservative
title_full Rapid cryopreservation of five mammalian and one mosquito cell line at -80°C while attached to flasks in a serum free cryopreservative
title_fullStr Rapid cryopreservation of five mammalian and one mosquito cell line at -80°C while attached to flasks in a serum free cryopreservative
title_full_unstemmed Rapid cryopreservation of five mammalian and one mosquito cell line at -80°C while attached to flasks in a serum free cryopreservative
title_short Rapid cryopreservation of five mammalian and one mosquito cell line at -80°C while attached to flasks in a serum free cryopreservative
title_sort rapid cryopreservation of five mammalian and one mosquito cell line at -80°c while attached to flasks in a serum free cryopreservative
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1190376/
https://www.ncbi.nlm.nih.gov/pubmed/16136221
http://dx.doi.org/10.1251/bpo102
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