Comparative proteomics using 2-D gel electrophoresis and mass spectrometry as tools to dissect stimulons and regulons in bacteria with sequenced or partially sequenced genomes

We propose two-dimensional gel electrophoresis (2-DE) and mass spectrometry to define the protein components of regulons and stimulons in bacteria, including those organisms where genome sequencing is still in progress. The basic 2-DE protocol allows high resolution and reproducibility and enables t...

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Detalles Bibliográficos
Autores principales: Encarnación, Sergio, Hernández, Magdalena, Martínez-Batallar, Gabriel, Contreras, Sandra, del Carmen Vargas, María, Mora, Jaime
Formato: Texto
Lenguaje:English
Publicado: Biological Procedures Online 2005
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1190382/
https://www.ncbi.nlm.nih.gov/pubmed/16145578
http://dx.doi.org/10.1251/bpo110

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