Cargando…

The influence of acetyl phosphate on DspA signalling in the Cyanobacterium Synechocystis sp. PCC6803

BACKGROUND: The dspA (hik33) gene, coding for a putative sensory histidine kinase, is conserved in plastids (ycf26) and cyanobacteria. It has been linked with a number of different stress responses in cyanobacteria. RESULTS: We constructed an insertional mutant of dspA (ycf26) in Synechocystis 6803....

Descripción completa

Detalles Bibliográficos
Autores principales: Morrison, S Shawn, Mullineaux, Conrad W, Ashby, Mark K
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2005
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1192802/
https://www.ncbi.nlm.nih.gov/pubmed/16076400
http://dx.doi.org/10.1186/1471-2180-5-47
_version_ 1782124816824795136
author Morrison, S Shawn
Mullineaux, Conrad W
Ashby, Mark K
author_facet Morrison, S Shawn
Mullineaux, Conrad W
Ashby, Mark K
author_sort Morrison, S Shawn
collection PubMed
description BACKGROUND: The dspA (hik33) gene, coding for a putative sensory histidine kinase, is conserved in plastids (ycf26) and cyanobacteria. It has been linked with a number of different stress responses in cyanobacteria. RESULTS: We constructed an insertional mutant of dspA (ycf26) in Synechocystis 6803. We found little phenotypic effect during nitrogen starvation. However, when the mutation was combined with deletion of the pta gene coding for phosphotransacetylase, a more significant phenotype was observed. Under nitrogen starvation, the pta/dspA double mutant degrades its phycobilisomes less than the wild type and still has about half of its chlorophyll-protein complexes. CONCLUSION: Our data indicates that acetyl-phosphate-dependent phosphorylation of response regulator(s) overlaps with DspA-dependent signalling of the degradation of chlorophyll-protein complexes (and to a lesser extent phycobilisomes) in Synechocystis 6803.
format Text
id pubmed-1192802
institution National Center for Biotechnology Information
language English
publishDate 2005
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-11928022005-08-27 The influence of acetyl phosphate on DspA signalling in the Cyanobacterium Synechocystis sp. PCC6803 Morrison, S Shawn Mullineaux, Conrad W Ashby, Mark K BMC Microbiol Research Article BACKGROUND: The dspA (hik33) gene, coding for a putative sensory histidine kinase, is conserved in plastids (ycf26) and cyanobacteria. It has been linked with a number of different stress responses in cyanobacteria. RESULTS: We constructed an insertional mutant of dspA (ycf26) in Synechocystis 6803. We found little phenotypic effect during nitrogen starvation. However, when the mutation was combined with deletion of the pta gene coding for phosphotransacetylase, a more significant phenotype was observed. Under nitrogen starvation, the pta/dspA double mutant degrades its phycobilisomes less than the wild type and still has about half of its chlorophyll-protein complexes. CONCLUSION: Our data indicates that acetyl-phosphate-dependent phosphorylation of response regulator(s) overlaps with DspA-dependent signalling of the degradation of chlorophyll-protein complexes (and to a lesser extent phycobilisomes) in Synechocystis 6803. BioMed Central 2005-08-02 /pmc/articles/PMC1192802/ /pubmed/16076400 http://dx.doi.org/10.1186/1471-2180-5-47 Text en Copyright © 2005 Morrison et al; licensee BioMed Central Ltd.
spellingShingle Research Article
Morrison, S Shawn
Mullineaux, Conrad W
Ashby, Mark K
The influence of acetyl phosphate on DspA signalling in the Cyanobacterium Synechocystis sp. PCC6803
title The influence of acetyl phosphate on DspA signalling in the Cyanobacterium Synechocystis sp. PCC6803
title_full The influence of acetyl phosphate on DspA signalling in the Cyanobacterium Synechocystis sp. PCC6803
title_fullStr The influence of acetyl phosphate on DspA signalling in the Cyanobacterium Synechocystis sp. PCC6803
title_full_unstemmed The influence of acetyl phosphate on DspA signalling in the Cyanobacterium Synechocystis sp. PCC6803
title_short The influence of acetyl phosphate on DspA signalling in the Cyanobacterium Synechocystis sp. PCC6803
title_sort influence of acetyl phosphate on dspa signalling in the cyanobacterium synechocystis sp. pcc6803
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1192802/
https://www.ncbi.nlm.nih.gov/pubmed/16076400
http://dx.doi.org/10.1186/1471-2180-5-47
work_keys_str_mv AT morrisonsshawn theinfluenceofacetylphosphateondspasignallinginthecyanobacteriumsynechocystissppcc6803
AT mullineauxconradw theinfluenceofacetylphosphateondspasignallinginthecyanobacteriumsynechocystissppcc6803
AT ashbymarkk theinfluenceofacetylphosphateondspasignallinginthecyanobacteriumsynechocystissppcc6803
AT morrisonsshawn influenceofacetylphosphateondspasignallinginthecyanobacteriumsynechocystissppcc6803
AT mullineauxconradw influenceofacetylphosphateondspasignallinginthecyanobacteriumsynechocystissppcc6803
AT ashbymarkk influenceofacetylphosphateondspasignallinginthecyanobacteriumsynechocystissppcc6803