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The influence of acetyl phosphate on DspA signalling in the Cyanobacterium Synechocystis sp. PCC6803
BACKGROUND: The dspA (hik33) gene, coding for a putative sensory histidine kinase, is conserved in plastids (ycf26) and cyanobacteria. It has been linked with a number of different stress responses in cyanobacteria. RESULTS: We constructed an insertional mutant of dspA (ycf26) in Synechocystis 6803....
Autores principales: | , , |
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Formato: | Texto |
Lenguaje: | English |
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BioMed Central
2005
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1192802/ https://www.ncbi.nlm.nih.gov/pubmed/16076400 http://dx.doi.org/10.1186/1471-2180-5-47 |
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author | Morrison, S Shawn Mullineaux, Conrad W Ashby, Mark K |
author_facet | Morrison, S Shawn Mullineaux, Conrad W Ashby, Mark K |
author_sort | Morrison, S Shawn |
collection | PubMed |
description | BACKGROUND: The dspA (hik33) gene, coding for a putative sensory histidine kinase, is conserved in plastids (ycf26) and cyanobacteria. It has been linked with a number of different stress responses in cyanobacteria. RESULTS: We constructed an insertional mutant of dspA (ycf26) in Synechocystis 6803. We found little phenotypic effect during nitrogen starvation. However, when the mutation was combined with deletion of the pta gene coding for phosphotransacetylase, a more significant phenotype was observed. Under nitrogen starvation, the pta/dspA double mutant degrades its phycobilisomes less than the wild type and still has about half of its chlorophyll-protein complexes. CONCLUSION: Our data indicates that acetyl-phosphate-dependent phosphorylation of response regulator(s) overlaps with DspA-dependent signalling of the degradation of chlorophyll-protein complexes (and to a lesser extent phycobilisomes) in Synechocystis 6803. |
format | Text |
id | pubmed-1192802 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2005 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-11928022005-08-27 The influence of acetyl phosphate on DspA signalling in the Cyanobacterium Synechocystis sp. PCC6803 Morrison, S Shawn Mullineaux, Conrad W Ashby, Mark K BMC Microbiol Research Article BACKGROUND: The dspA (hik33) gene, coding for a putative sensory histidine kinase, is conserved in plastids (ycf26) and cyanobacteria. It has been linked with a number of different stress responses in cyanobacteria. RESULTS: We constructed an insertional mutant of dspA (ycf26) in Synechocystis 6803. We found little phenotypic effect during nitrogen starvation. However, when the mutation was combined with deletion of the pta gene coding for phosphotransacetylase, a more significant phenotype was observed. Under nitrogen starvation, the pta/dspA double mutant degrades its phycobilisomes less than the wild type and still has about half of its chlorophyll-protein complexes. CONCLUSION: Our data indicates that acetyl-phosphate-dependent phosphorylation of response regulator(s) overlaps with DspA-dependent signalling of the degradation of chlorophyll-protein complexes (and to a lesser extent phycobilisomes) in Synechocystis 6803. BioMed Central 2005-08-02 /pmc/articles/PMC1192802/ /pubmed/16076400 http://dx.doi.org/10.1186/1471-2180-5-47 Text en Copyright © 2005 Morrison et al; licensee BioMed Central Ltd. |
spellingShingle | Research Article Morrison, S Shawn Mullineaux, Conrad W Ashby, Mark K The influence of acetyl phosphate on DspA signalling in the Cyanobacterium Synechocystis sp. PCC6803 |
title | The influence of acetyl phosphate on DspA signalling in the Cyanobacterium Synechocystis sp. PCC6803 |
title_full | The influence of acetyl phosphate on DspA signalling in the Cyanobacterium Synechocystis sp. PCC6803 |
title_fullStr | The influence of acetyl phosphate on DspA signalling in the Cyanobacterium Synechocystis sp. PCC6803 |
title_full_unstemmed | The influence of acetyl phosphate on DspA signalling in the Cyanobacterium Synechocystis sp. PCC6803 |
title_short | The influence of acetyl phosphate on DspA signalling in the Cyanobacterium Synechocystis sp. PCC6803 |
title_sort | influence of acetyl phosphate on dspa signalling in the cyanobacterium synechocystis sp. pcc6803 |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1192802/ https://www.ncbi.nlm.nih.gov/pubmed/16076400 http://dx.doi.org/10.1186/1471-2180-5-47 |
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