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A molecular analysis by gene expression profiling reveals Bik/NBK overexpression in sporadic breast tumor samples of Mexican females

BACKGROUND: Breast cancer is one of the most frequent causes of death in Mexican women over 35 years of age. At molecular level, changes in many genetic networks have been reported as associated with this neoplasia. To analyze these changes, we determined gene expression profiles of tumors from Mexi...

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Autores principales: García, Normand, Salamanca, Fabio, Astudillo-de la Vega, Horacio, Curiel-Quesada, Everardo, Alvarado, Isabel, Peñaloza, Rosenda, Arenas, Diego
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2005
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1198222/
https://www.ncbi.nlm.nih.gov/pubmed/16060964
http://dx.doi.org/10.1186/1471-2407-5-93
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author García, Normand
Salamanca, Fabio
Astudillo-de la Vega, Horacio
Curiel-Quesada, Everardo
Alvarado, Isabel
Peñaloza, Rosenda
Arenas, Diego
author_facet García, Normand
Salamanca, Fabio
Astudillo-de la Vega, Horacio
Curiel-Quesada, Everardo
Alvarado, Isabel
Peñaloza, Rosenda
Arenas, Diego
author_sort García, Normand
collection PubMed
description BACKGROUND: Breast cancer is one of the most frequent causes of death in Mexican women over 35 years of age. At molecular level, changes in many genetic networks have been reported as associated with this neoplasia. To analyze these changes, we determined gene expression profiles of tumors from Mexican women with breast cancer at different stages and compared these with those of normal breast tissue samples. METHODS: (32)P-radiolabeled cDNA was synthesized by reverse transcription of mRNA from fresh sporadic breast tumor biopsies, as well as normal breast tissue. cDNA probes were hybridized to microarrays and expression levels registered using a phosphorimager. Expression levels of some genes were validated by real time RT-PCR and immunohistochemical assays. RESULTS: We identified two subgroups of tumors according to their expression profiles, probably related with cancer progression. Ten genes, unexpressed in normal tissue, were turned on in some tumors. We found consistent high expression of Bik gene in 14/15 tumors with predominant cytoplasmic distribution. CONCLUSION: Recently, the product of the Bik gene has been associated with tumoral reversion in different neoplasic cell lines, and was proposed as therapy to induce apoptosis in cancers, including breast tumors. Even though a relationship among genes, for example those from a particular pathway, can be observed through microarrays, this relationship might not be sufficient to assign a definitive role to Bik in development and progression of the neoplasia. The findings herein reported deserve further investigation.
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spelling pubmed-11982222005-09-03 A molecular analysis by gene expression profiling reveals Bik/NBK overexpression in sporadic breast tumor samples of Mexican females García, Normand Salamanca, Fabio Astudillo-de la Vega, Horacio Curiel-Quesada, Everardo Alvarado, Isabel Peñaloza, Rosenda Arenas, Diego BMC Cancer Research Article BACKGROUND: Breast cancer is one of the most frequent causes of death in Mexican women over 35 years of age. At molecular level, changes in many genetic networks have been reported as associated with this neoplasia. To analyze these changes, we determined gene expression profiles of tumors from Mexican women with breast cancer at different stages and compared these with those of normal breast tissue samples. METHODS: (32)P-radiolabeled cDNA was synthesized by reverse transcription of mRNA from fresh sporadic breast tumor biopsies, as well as normal breast tissue. cDNA probes were hybridized to microarrays and expression levels registered using a phosphorimager. Expression levels of some genes were validated by real time RT-PCR and immunohistochemical assays. RESULTS: We identified two subgroups of tumors according to their expression profiles, probably related with cancer progression. Ten genes, unexpressed in normal tissue, were turned on in some tumors. We found consistent high expression of Bik gene in 14/15 tumors with predominant cytoplasmic distribution. CONCLUSION: Recently, the product of the Bik gene has been associated with tumoral reversion in different neoplasic cell lines, and was proposed as therapy to induce apoptosis in cancers, including breast tumors. Even though a relationship among genes, for example those from a particular pathway, can be observed through microarrays, this relationship might not be sufficient to assign a definitive role to Bik in development and progression of the neoplasia. The findings herein reported deserve further investigation. BioMed Central 2005-08-01 /pmc/articles/PMC1198222/ /pubmed/16060964 http://dx.doi.org/10.1186/1471-2407-5-93 Text en Copyright © 2005 García et al; licensee BioMed Central Ltd.
spellingShingle Research Article
García, Normand
Salamanca, Fabio
Astudillo-de la Vega, Horacio
Curiel-Quesada, Everardo
Alvarado, Isabel
Peñaloza, Rosenda
Arenas, Diego
A molecular analysis by gene expression profiling reveals Bik/NBK overexpression in sporadic breast tumor samples of Mexican females
title A molecular analysis by gene expression profiling reveals Bik/NBK overexpression in sporadic breast tumor samples of Mexican females
title_full A molecular analysis by gene expression profiling reveals Bik/NBK overexpression in sporadic breast tumor samples of Mexican females
title_fullStr A molecular analysis by gene expression profiling reveals Bik/NBK overexpression in sporadic breast tumor samples of Mexican females
title_full_unstemmed A molecular analysis by gene expression profiling reveals Bik/NBK overexpression in sporadic breast tumor samples of Mexican females
title_short A molecular analysis by gene expression profiling reveals Bik/NBK overexpression in sporadic breast tumor samples of Mexican females
title_sort molecular analysis by gene expression profiling reveals bik/nbk overexpression in sporadic breast tumor samples of mexican females
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1198222/
https://www.ncbi.nlm.nih.gov/pubmed/16060964
http://dx.doi.org/10.1186/1471-2407-5-93
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