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Expression of TGF-β1 and β3 but not apoptosis factors relates to flow-induced aortic enlargement
BACKGROUND: Cell proliferation and apoptosis are both involved in arterial wall remodeling. Increase in blood flow induces arterial enlargement. The molecular basis of flow-induced remodeling in large elastic arteries is largely unknown. METHODS: An aortocaval fistula (ACF) model in rats was used to...
Autores principales: | , , , , |
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Formato: | Texto |
Lenguaje: | English |
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BioMed Central
2002
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC119850/ https://www.ncbi.nlm.nih.gov/pubmed/12150715 |
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author | Xu, Chengpei Lee, Sheila Shu, Chang Masuda, Hirotake Zarins, Christopher K |
author_facet | Xu, Chengpei Lee, Sheila Shu, Chang Masuda, Hirotake Zarins, Christopher K |
author_sort | Xu, Chengpei |
collection | PubMed |
description | BACKGROUND: Cell proliferation and apoptosis are both involved in arterial wall remodeling. Increase in blood flow induces arterial enlargement. The molecular basis of flow-induced remodeling in large elastic arteries is largely unknown. METHODS: An aortocaval fistula (ACF) model in rats was used to induce enlargement in the abdominal aorta. Aortic gene expression of transforming growth factors beta (TGF-β) and apoptosis-related factors was assessed at 1 and 3 days and 1, 2, 4, and 8 weeks. Expression levels were determined using a ribonuclease protection assay and western blotting. Cell proliferation and apoptosis were analyzed using BrdU incorporation and TUNEL techniques. RESULTS: Blood flow increased 5-fold immediately after ACF (P<0.05). Lumen diameter of the aorta was 30% and 75% larger at 2 and 8 weeks respectively than those of controls (P<0.05). mRNA levels of TGF-β1 and TGF-β3 increased after ACF, peaked at 3 days (P<0.05) and returned to normal level at 1 week and thereafter. Western blotting showed enhanced expression of TGF-β1 at 3 days and TGF-β3 at 1 and 3 days and 1 week (P<0.05). mRNA levels of Bcl-xS initially decreased at 1 day, 3 days and 1 week, followed a return to baseline level at 2 weeks. Cell proliferation was observed at all time points after ACF (P<0.001 vs. controls) with proliferation in endothelial cells more significant than smooth muscle cells. Apoptosis was not significant. CONCLUSIONS: Gene expression of TGF-β1 and β3 precedes arterial enlargement. Expression of apoptosis related factors is little regulated in the early stage of the flow-induced arterial remodeling. |
format | Text |
id | pubmed-119850 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2002 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-1198502002-09-04 Expression of TGF-β1 and β3 but not apoptosis factors relates to flow-induced aortic enlargement Xu, Chengpei Lee, Sheila Shu, Chang Masuda, Hirotake Zarins, Christopher K BMC Cardiovasc Disord Research Article BACKGROUND: Cell proliferation and apoptosis are both involved in arterial wall remodeling. Increase in blood flow induces arterial enlargement. The molecular basis of flow-induced remodeling in large elastic arteries is largely unknown. METHODS: An aortocaval fistula (ACF) model in rats was used to induce enlargement in the abdominal aorta. Aortic gene expression of transforming growth factors beta (TGF-β) and apoptosis-related factors was assessed at 1 and 3 days and 1, 2, 4, and 8 weeks. Expression levels were determined using a ribonuclease protection assay and western blotting. Cell proliferation and apoptosis were analyzed using BrdU incorporation and TUNEL techniques. RESULTS: Blood flow increased 5-fold immediately after ACF (P<0.05). Lumen diameter of the aorta was 30% and 75% larger at 2 and 8 weeks respectively than those of controls (P<0.05). mRNA levels of TGF-β1 and TGF-β3 increased after ACF, peaked at 3 days (P<0.05) and returned to normal level at 1 week and thereafter. Western blotting showed enhanced expression of TGF-β1 at 3 days and TGF-β3 at 1 and 3 days and 1 week (P<0.05). mRNA levels of Bcl-xS initially decreased at 1 day, 3 days and 1 week, followed a return to baseline level at 2 weeks. Cell proliferation was observed at all time points after ACF (P<0.001 vs. controls) with proliferation in endothelial cells more significant than smooth muscle cells. Apoptosis was not significant. CONCLUSIONS: Gene expression of TGF-β1 and β3 precedes arterial enlargement. Expression of apoptosis related factors is little regulated in the early stage of the flow-induced arterial remodeling. BioMed Central 2002-07-31 /pmc/articles/PMC119850/ /pubmed/12150715 Text en Copyright ©2002 Xu et al; licensee BioMed Central Ltd. This article is published in Open Access: verbatim copying and redistribution of this article are permitted in all media for any non-commercial purpose, provided this notice is preserved along with the article's original URL. |
spellingShingle | Research Article Xu, Chengpei Lee, Sheila Shu, Chang Masuda, Hirotake Zarins, Christopher K Expression of TGF-β1 and β3 but not apoptosis factors relates to flow-induced aortic enlargement |
title | Expression of TGF-β1 and β3 but not apoptosis factors relates to flow-induced aortic enlargement |
title_full | Expression of TGF-β1 and β3 but not apoptosis factors relates to flow-induced aortic enlargement |
title_fullStr | Expression of TGF-β1 and β3 but not apoptosis factors relates to flow-induced aortic enlargement |
title_full_unstemmed | Expression of TGF-β1 and β3 but not apoptosis factors relates to flow-induced aortic enlargement |
title_short | Expression of TGF-β1 and β3 but not apoptosis factors relates to flow-induced aortic enlargement |
title_sort | expression of tgf-β1 and β3 but not apoptosis factors relates to flow-induced aortic enlargement |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC119850/ https://www.ncbi.nlm.nih.gov/pubmed/12150715 |
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