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Crystal structure of nitrogen regulatory protein IIA(Ntr )from Neisseria meningitidis

BACKGROUND: The NMB0736 gene of Neisseria meningitidis serogroup B strain MC58 encodes the putative nitrogen regulatory protein, IIA(Ntr )(abbreviated to NM-IIA(Ntr)). The homologous protein present in Escherichia coli is implicated in the control of nitrogen assimilation. As part of a structural pr...

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Autores principales: Ren, Jingshan, Sainsbury, Sarah, Berrow, Nick S, Alderton, David, Nettleship, Joanne E, Stammers, David K, Saunders, Nigel J, Owens, Raymond J
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2005
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1201152/
https://www.ncbi.nlm.nih.gov/pubmed/16092953
http://dx.doi.org/10.1186/1472-6807-5-13
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author Ren, Jingshan
Sainsbury, Sarah
Berrow, Nick S
Alderton, David
Nettleship, Joanne E
Stammers, David K
Saunders, Nigel J
Owens, Raymond J
author_facet Ren, Jingshan
Sainsbury, Sarah
Berrow, Nick S
Alderton, David
Nettleship, Joanne E
Stammers, David K
Saunders, Nigel J
Owens, Raymond J
author_sort Ren, Jingshan
collection PubMed
description BACKGROUND: The NMB0736 gene of Neisseria meningitidis serogroup B strain MC58 encodes the putative nitrogen regulatory protein, IIA(Ntr )(abbreviated to NM-IIA(Ntr)). The homologous protein present in Escherichia coli is implicated in the control of nitrogen assimilation. As part of a structural proteomics approach to the study of pathogenic Neisseria spp., we have selected this protein for structure determination by X-ray crystallography. RESULTS: The NM-IIA(Ntr )was over-expressed in E. coli and was shown to be partially mono-phosphorylated, as assessed by mass spectrometry of the purified protein. Crystals of un-phosphorylated protein were obtained and diffraction data collected to 2.5 Å resolution. The structure of NM-IIA(Ntr )was solved by molecular replacement using the coordinates of the E. coli nitrogen regulatory protein IIA(ntr )[PDB: 1A6J] as the starting model. The overall fold of the Neisseria enzyme shows a high degree of similarity to the IIA(Ntr )from E. coli, and the position of the phosphoryl acceptor histidine residue (H67) is conserved. The orientation of an adjacent arginine residue (R69) suggests that it may also be involved in coordinating the phosphate group. Comparison of the structure with that of E. coli IIA(mtl )complexed with HPr [PDB: 1J6T] indicates that NM-IIA(Ntr )binds in a similar way to the HPr-like enzyme in Neisseria. CONCLUSION: The structure of NM-IIA(Ntr )confirms its assignment as a homologue of the IIA(Ntr )proteins found in a range of other Gram-negative bacteria. We conclude that the NM- IIA(Ntr )protein functions as part of a phosphorylation cascade which, in contrast to E. coli, shares the upstream phosphotransfer protein with the sugar uptake phosphoenolpyruvate:sugar phosphotransferase system (PTS), but in common with E. coli has a distinct downstream effector mechanism.
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spelling pubmed-12011522005-09-10 Crystal structure of nitrogen regulatory protein IIA(Ntr )from Neisseria meningitidis Ren, Jingshan Sainsbury, Sarah Berrow, Nick S Alderton, David Nettleship, Joanne E Stammers, David K Saunders, Nigel J Owens, Raymond J BMC Struct Biol Research Article BACKGROUND: The NMB0736 gene of Neisseria meningitidis serogroup B strain MC58 encodes the putative nitrogen regulatory protein, IIA(Ntr )(abbreviated to NM-IIA(Ntr)). The homologous protein present in Escherichia coli is implicated in the control of nitrogen assimilation. As part of a structural proteomics approach to the study of pathogenic Neisseria spp., we have selected this protein for structure determination by X-ray crystallography. RESULTS: The NM-IIA(Ntr )was over-expressed in E. coli and was shown to be partially mono-phosphorylated, as assessed by mass spectrometry of the purified protein. Crystals of un-phosphorylated protein were obtained and diffraction data collected to 2.5 Å resolution. The structure of NM-IIA(Ntr )was solved by molecular replacement using the coordinates of the E. coli nitrogen regulatory protein IIA(ntr )[PDB: 1A6J] as the starting model. The overall fold of the Neisseria enzyme shows a high degree of similarity to the IIA(Ntr )from E. coli, and the position of the phosphoryl acceptor histidine residue (H67) is conserved. The orientation of an adjacent arginine residue (R69) suggests that it may also be involved in coordinating the phosphate group. Comparison of the structure with that of E. coli IIA(mtl )complexed with HPr [PDB: 1J6T] indicates that NM-IIA(Ntr )binds in a similar way to the HPr-like enzyme in Neisseria. CONCLUSION: The structure of NM-IIA(Ntr )confirms its assignment as a homologue of the IIA(Ntr )proteins found in a range of other Gram-negative bacteria. We conclude that the NM- IIA(Ntr )protein functions as part of a phosphorylation cascade which, in contrast to E. coli, shares the upstream phosphotransfer protein with the sugar uptake phosphoenolpyruvate:sugar phosphotransferase system (PTS), but in common with E. coli has a distinct downstream effector mechanism. BioMed Central 2005-08-10 /pmc/articles/PMC1201152/ /pubmed/16092953 http://dx.doi.org/10.1186/1472-6807-5-13 Text en Copyright ©2005 Ren et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Ren, Jingshan
Sainsbury, Sarah
Berrow, Nick S
Alderton, David
Nettleship, Joanne E
Stammers, David K
Saunders, Nigel J
Owens, Raymond J
Crystal structure of nitrogen regulatory protein IIA(Ntr )from Neisseria meningitidis
title Crystal structure of nitrogen regulatory protein IIA(Ntr )from Neisseria meningitidis
title_full Crystal structure of nitrogen regulatory protein IIA(Ntr )from Neisseria meningitidis
title_fullStr Crystal structure of nitrogen regulatory protein IIA(Ntr )from Neisseria meningitidis
title_full_unstemmed Crystal structure of nitrogen regulatory protein IIA(Ntr )from Neisseria meningitidis
title_short Crystal structure of nitrogen regulatory protein IIA(Ntr )from Neisseria meningitidis
title_sort crystal structure of nitrogen regulatory protein iia(ntr )from neisseria meningitidis
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1201152/
https://www.ncbi.nlm.nih.gov/pubmed/16092953
http://dx.doi.org/10.1186/1472-6807-5-13
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